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Related Experiment Video

Updated: Mar 16, 2026

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
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Aptamer-based fiber sensor for thrombin detection.

Luís Coelho1, José Manuel Marques Martins de Almeida2, José Luís Santos1

  • 1University of Porto, CAP/INESC TEC-Technology and Science and FCUP-Faculty of Sciences, Rua do Campo Alegre, 4150-179 Porto, Portugal.

Journal of Biomedical Optics
|August 23, 2016
PubMed
Summary

This study compares two optical fiber sensors for detecting thrombin using aptamer binding. Surface plasmon resonance (SPR) sensors demonstrated higher sensitivity and resolution for thrombin detection compared to long period gratings.

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Thrombin detection is crucial for diagnosing and monitoring various medical conditions.
  • Optical fiber sensors offer sensitive and label-free detection methods.
  • Aptamer-based biosensing provides high specificity for target biomolecules.

Purpose of the Study:

  • To compare the performance of two optical fiber-based sensor configurations for thrombin detection.
  • To evaluate aptamer-functionalized long period gratings (LPGs) and surface plasmon resonance (SPR) devices.
  • To determine the sensitivity and resolution of each sensor for thrombin in the 10-100 nM range.

Main Methods:

  • Fabrication and functionalization of LPGs with titanium dioxide and SPR devices with gold/titanium dioxide multilayers.
  • Functionalization of sensor surfaces with aptamers for specific thrombin binding.
  • Comparative analysis of sensor performance in transmission mode using varying thrombin concentrations.

Main Results:

  • The SPR device exhibited significantly higher sensitivity to refractive index changes (3100 nm/RIU) compared to the LPG (20x lower).
  • Successful detection of 10 nM thrombin was achieved with the SPR sensor, showing a 3.5 nm wavelength shift.
  • The SPR sensor achieved a resolution of 0.54 nM for thrombin detection.

Conclusions:

  • Optical fiber SPR sensors functionalized with aptamers offer superior performance for sensitive thrombin detection.
  • SPR-based biosensors show promise for clinical applications requiring rapid and accurate thrombin quantification.
  • The developed SPR sensor provides a high-resolution platform for aptamer-based biomolecule detection.