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Related Experiment Video

Updated: Mar 16, 2026

Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells
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A ratiometric fluorescent sensor for the mitochondrial copper pool.

Clara Shen1, Jacek L Kolanowski, Carmen M-N Tran

  • 1School of Chemistry, The University of Sydney, NSW 2006, Australia. Elizabeth.new@sydney.edu.au.

Metallomics : Integrated Biometal Science
|August 24, 2016
PubMed
Summary
This summary is machine-generated.

Researchers developed a new sensor, InCCu1, to track mitochondrial copper. Cisplatin treatment was found to impair cellular copper uptake in mitochondria.

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Bioimaging

Background:

  • Copper is essential for cellular processes including defense and growth.
  • Mitochondria are key organelles involved in cellular metabolism and function.
  • Accurate measurement of intracellular copper is crucial for understanding its role in health and disease.

Purpose of the Study:

  • To develop a novel ratiometric fluorescent sensor for detecting mitochondrial copper(I) ions.
  • To investigate the impact of cisplatin treatment on mitochondrial copper accumulation.

Main Methods:

  • Development of InCCu1, a ratiometric fluorescent probe sensitive to Cu(i).
  • Application of InCCu1 in fluorescence microscopy and flow cytometry.
  • Analysis of copper levels in mitochondria of cisplatin-treated cells.

Main Results:

  • The InCCu1 sensor exhibits a ratiometric change from red to blue emission upon binding to Cu(i).
  • Microscopy and flow cytometry revealed that cisplatin-treated cells show reduced mitochondrial copper accumulation.
  • This indicates an impaired ability of these cells to uptake copper into mitochondria.

Conclusions:

  • InCCu1 is an effective tool for visualizing and quantifying mitochondrial copper in live cells.
  • Cisplatin disrupts normal mitochondrial copper homeostasis.
  • The findings highlight a potential mechanism of cisplatin-induced cellular dysfunction related to copper metabolism.