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Local anesthetics induce autophagy in young permanent tooth pulp cells
1Department of Cariology, Endodontology and Operative Dentistry, Peking University School and Hospital of Stomatology, 22 South Zhongguancun Avenue, Haidian District, Beijing, P.R. China; Peninsula Dental School, Plymouth University Peninsula Schools of Medicine and Dentistry, 16 Research Way, Plymouth, UK.
Cell Death Discovery
|August 24, 2016
Summary
Local anesthetics impact tooth pulp cell proliferation, potentially affecting tooth development. Early intervention with a V-ATPase inhibitor may partially reverse these effects on dental stem cells.
Area of Science:
- Dental research
- Cell biology
- Developmental biology
Background:
- Dental pulp cells are crucial for tooth development, repair, and regeneration, serving as a vital stem cell source.
- Local anesthetics are frequently used in clinical settings and have been implicated in interfering with permanent tooth development and causing tooth agenesis via unknown mechanisms.
Purpose of the Study:
- To investigate the effects of commonly used local anesthetics on human tooth pulp cell proliferation.
- To identify molecular pathways and downstream targets affected by these local anesthetics.
Main Methods:
- Utilized a pig model and human young permanent tooth pulp cells.
- Performed molecular pathway profiling to analyze cellular responses to local anesthetics.
- Investigated the potential for recovery using a V-ATPase inhibitor.
Main Results:
- Local anesthetics were found to affect tooth pulp cell proliferation.
- LC3II was identified as an early induced molecule, with p62 as a common downstream target for all tested drugs.
- Early intervention with a V-ATPase inhibitor demonstrated partial recovery of the affected cells.
Conclusions:
- Local anesthetics can negatively impact tooth cell growth.
- These findings provide novel insights into the potential mechanisms by which local anesthetics may affect tooth development and highlight the importance of early intervention.
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