Related Experiment Video
Updated: Mar 16, 2026
Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
Published on: March 30, 2019
miR-297 acts as an oncogene by targeting GPC5 in lung adenocarcinoma
Yunchuan Sun1, Jianyong Zhao1, Xiaoming Yin1
1Department of Radiation and Chemotherapy, Hebei Province Cangzhou Hospital of Integrated Traditional and Western Medicine, Cangzhou, Hebei, 061000, China.
Objectives:
Emerging studies have demonstrated that microRNAs (miRNAs) play crucial roles in carcinogenesis of many developing human tumours. However, the functions and mechanisms of miR-297 in lung cancer have, up to now, been largely undefined.
Materials And Methods:
Here, miR-297 expression was measured in lung adenocarcinoma tissues and cell lines, using qRT-PCR. Lung adenocarcinoma cell line was treated with an miR-297 mimic. MTT and colony analysis were performed to detect cell proliferation and colony formation. The direct target gene of miR-297 was assessed by qRT-PCR, Western blotting and luciferase assays.
Results:
We demonstrated that miR-297 expression was upregulated in lung adenocarcinomas compared to adjacent normal tissues. Expression of miR-297 was also upregulated in tested lung adenocarcinoma cell lines. Ectopic expression of miR-297 enhanced lung adenocarcinoma cell proliferation and colony formation. Furthermore, overexpression of miR-297 promoted cell migration and invasion. In addition, we identified Glypican-5 (GPC5) as a direct target gene of miR-297 in lung adenocarcinoma cells. Expression of GPC5 was downregulated in both lung adenocarcinoma tissues and cell lines. Moreover, expression of GPC5 was inversely associated with expression of miR-297 in lung adenocarcinoma tissues.
Conclusions:
These results suggest that miR-297 acted as an oncogenic miRNA, partly by targeting GPC5, adenocarcinoma of the lung.
Insights
MicroRNA-297 (miR-297) is upregulated in lung adenocarcinoma, promoting cancer cell growth and invasion. It targets Glypican-5 (GPC5), suggesting miR-297 acts as an oncogene in this cancer.
Area of Science:
- Oncology
- Molecular Biology
- Gene Regulation
Background:
- MicroRNAs (miRNAs) are key regulators in cancer development.
- The specific role of miR-297 in lung adenocarcinoma remains largely unknown.
Purpose of the Study:
- To investigate the function and mechanism of miR-297 in lung adenocarcinoma.
- To identify the target genes of miR-297 in this cancer type.
Main Methods:
- Quantitative reverse transcription polymerase chain reaction (qRT-PCR) to measure miR-297 and GPC5 expression.
- Cell proliferation and colony formation assays (MTT, colony analysis).
- Luciferase assays, Western blotting to confirm direct targeting of GPC5 by miR-297.
Main Results:
- miR-297 expression was significantly higher in lung adenocarcinoma tissues and cell lines.
- Overexpression of miR-297 increased lung adenocarcinoma cell proliferation, migration, and invasion.
- Glypican-5 (GPC5) was identified as a direct target of miR-297 and was downregulated in tumors.
Conclusions:
- miR-297 functions as an oncogenic miRNA in lung adenocarcinoma.
- miR-297 promotes cancer progression, partly through the downregulation of its target gene GPC5.
More Related Videos
13:18Network Pharmacology Prediction and Experimental Validation of Trichosanthes-Fritillaria thunbergii Action Mechanism Against Lung Adenocarcinoma
Published on: March 3, 2023
06:03Orthotopic Transplantation of Syngeneic Lung Adenocarcinoma Cells to Study PD-L1 Expression
Published on: January 19, 2019
Related Concept Videos
02:23Abnormal Proliferation
02:38Mitogens and the Cell Cycle
01:20The Retinoblastoma Gene
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
03:03mTOR Signaling and Cancer Progression
The mTOR pathway or the...