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Updated: Mar 16, 2026
Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
Published on: March 3, 2023
Detection of Multi-drug Resistant Acinetobacter Lwoffii Isolated from Soil of Mink Farm
Na Sun1, Yong Jun Wen1, Shu Qin Zhang1
1State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animals and Plants,Chinese Academy of Agricultural Sciences CAAS, Changchun 130112, Jilin, China.
Abstract:
There were 4 Acinetobacter lwoffii obtained from soil samples. The antimicrobial susceptibility of the strains to 16 antimicrobial agents was investigated using K-B method. Three isolates showed the multi-drug resistance. The presence of resistance genes and integrons was determined using PCR. The aadA1, aac(3')-IIc, aph(3')-VII, aac(6')-Ib, sul2, cat2, floR, and tet(K) genes were detected, respectively. Three class 1 integrons were obtained. The arr-3-aacA4 and blaPSE-1 gene cassette, which cause resistance to aminoglycoside and beta-lactamase antibiotics. Our results reported the detection of multi-drug resistant and carried resistant genes Acinetobacter lwoffii from soil. The findings suggested that we should pay close attention to the prevalence of multi-drug resistant bacterial species of environment.
Insights
Soil samples revealed multidrug-resistant Acinetobacter lwoffii carrying various resistance genes and integrons. This highlights the environmental prevalence of antibiotic resistance, necessitating increased attention.
Area of Science:
- Environmental microbiology
- Molecular biology
- Antimicrobial resistance
Background:
- Acinetobacter lwoffii isolates were obtained from soil samples.
- Antimicrobial susceptibility testing was performed against 16 agents using the K-B method.
- Three of the four Acinetobacter lwoffii isolates exhibited multidrug resistance.
Discussion:
- Polymerase Chain Reaction (PCR) was employed to detect specific resistance genes, including aadA1, aac(3')-IIc, aph(3')-VII, aac(6')-Ib, sul2, cat2, floR, and tet(K).
- Three class 1 integrons were identified, along with the arr-3-aacA4 and blaPSE-1 gene cassettes, conferring resistance to aminoglycoside and beta-lactamase antibiotics.
- The study successfully detected multidrug-resistant Acinetobacter lwoffii strains harboring resistance genes in soil environments.
Key Insights:
- Detection of multiple antibiotic resistance genes (e.g., aadA1, tet(K)) in soil-dwelling Acinetobacter lwoffii.
- Identification of class 1 integrons and specific gene cassettes (arr-3-aacA4, blaPSE-1) linked to multidrug resistance.
- Confirmation of Acinetobacter lwoffii as a reservoir for antibiotic resistance determinants in the environment.
Outlook:
- The findings underscore the importance of monitoring environmental bacteria for multidrug resistance.
- Further research is needed to understand the transmission dynamics of antibiotic resistance genes in soil ecosystems.
- Environmental surveillance is crucial for mitigating the spread of antimicrobial resistance.
