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Concurrent Quantification of Cellular and Extracellular Components of Biofilms
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High-Velocity Microsprays Enhance Antimicrobial Activity in Streptococcus mutans Biofilms.

S Fabbri1, D A Johnston2, A Rmaile3

  • 1National Centre for Advanced Tribology at Southampton (nCATS), University of Southampton, Southampton, UK stefaniafabbri1987@gmail.com.

Journal of Dental Research
|August 25, 2016
PubMed
Summary
This summary is machine-generated.

High-velocity water microsprays effectively deliver antimicrobials into Streptococcus mutans biofilms, improving caries prevention. Optimized impact angles enhance microbead delivery and bacterial killing compared to traditional methods.

Keywords:
antimicrobials/antimicrobial resistancecaries detection/diagnosis/preventiondental hygienedrug deliveryoral hygieneplaque/plaque biofilms

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Area of Science:

  • Oral microbiology
  • Biomaterials science
  • Biofluid dynamics

Background:

  • Streptococcus mutans biofilms contribute to dental caries development.
  • Biofilm structure impedes antimicrobial agent penetration.
  • Novel delivery methods are needed to overcome biofilm resistance.

Purpose of the Study:

  • To assess high-velocity water microsprays for enhanced antimicrobial delivery into S. mutans biofilms.
  • To compare microspray delivery with diffusive transport and mouthwash methods.
  • To investigate the effect of impact angle on delivery efficiency.

Main Methods:

  • Exposed 3-day-old S. mutans biofilms to microsprays with 90° or 30° impact angles.
  • Used 1-µm tracer beads and antimicrobial solutions (chlorhexidine/cetylpyridinium chloride).
  • Analyzed bead penetration depth and bacterial killing zones using confocal microscopy and viability staining.

Main Results:

  • Microsprays delivered significantly more microbeads deeper into biofilms than diffusion or mouthwash.
  • Microsprays achieved greater antimicrobial penetration and bacterial killing depth.
  • A 30° impact angle delivered 16x more microbeads and increased bacterial killing by ~20% compared to a 90° angle.

Conclusions:

  • High-velocity water microsprays are effective for delivering microparticles and antimicrobials into S. mutans biofilms.
  • Shear stresses and physical restructuring enhance delivery, suggesting a novel therapeutic approach.
  • Optimizing impact angle is crucial for maximizing biofilm disruption and antimicrobial efficacy.