Fine structure of synaptic sites and circuits in mushroom bodies of insect brains

In the insect brain, mushroom bodies represent a prominent central neuropil for multisensory integration and, crucially, for learning and memory. For this reason, special attention has been focused on its small chemical synapses. Early studies on synaptic types and their distribution, using conventional electron microscopy, and recent publications have resolved basic features of synaptic circuits. More recent studies, using experimental methods for resolving neurons, such as immunocytochemistry, genetic labelling, high resolution confocal microscopy and more advanced electron microscopy, have revealed many new details about the fine structure and molecular contents of identifiable neurons of mushroom bodies and has led to more refined modelling of functional organisation. Synaptic circuitries have been described in most detail for the calyces. In contrast, the mushroom bodies' columnar peduncle and lobes have been explored to a lesser degree. In dissecting local microcircuits, the scientist is confronted with complex neuronal compartmentalisation and specific synaptic arrangements. This article reviews classical and modern studies on the fine structure of synapses and their networks in mushroom bodies across several insect species.