Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

8.8K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
8.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Elevated IL-6 receptor expression on CD4+ T cells contributes to the increased Th17 responses in patients with chronic hepatitis B.

Virology journal·2011
Same author

Neurochemical plasticity of nitric oxide synthase isoforms in neurogenic detrusor overactivity after spinal cord injury.

Neurochemical research·2011
Same author

[Clinical significance of 5-HT and DA levels in serum and cerebrospinal fluid of the patients with delayed encephalopathy after acute carbon monoxide poisoning].

Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases·2011
Same author

Reconstitution of lysosomal NAADP-TRP-ML1 signaling pathway and its function in TRP-ML1(-/-) cells.

American journal of physiology. Cell physiology·2011
Same author

[The association between HBV genotyping and clinical characteristics and expression of TH1/TH2 cytokines].

Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology·2011
Same author

Bis[5-(2-pyrid-yl)pyrazine-2-carbonitrile]-silver(I) tetra-fluorido-borate.

Acta crystallographica. Section E, Structure reports online·2011

Related Experiment Video

Updated: Mar 16, 2026

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
07:58

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

Published on: September 19, 2018

7.2K

A method for identifying discriminative isoform-specific peptides for clinical proteomics application.

Fan Zhang1, Jake Y Chen2,3,4,5

  • 1Department of Molecular and Medical Genetics, University of North Texas Health Science Center, Fort Worth, TX, 76107, USA.

BMC Genomics
|August 25, 2016
PubMed
Summary

Protein isoforms are emerging as crucial early diagnostic biomarkers for cancer. This study introduces a novel peptidomics approach to identify cancer-specific protein isoforms, enhancing early breast cancer detection and clinical proteomics applications.

More Related Videos

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry
11:54

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

Published on: March 23, 2020

10.5K
Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling
11:53

Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling

Published on: July 1, 2014

16.9K

Related Experiment Videos

Last Updated: Mar 16, 2026

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
07:58

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

Published on: September 19, 2018

7.2K
Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry
11:54

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

Published on: March 23, 2020

10.5K
Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling
11:53

Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling

Published on: July 1, 2014

16.9K

Area of Science:

  • Proteomics
  • Biomarker Discovery
  • Molecular Diagnostics

Background:

  • Protein isoforms, arising from SNPs, alternative splicing, or PTMs, are critical in tumorigenesis, diagnosis, and prognosis.
  • Traditional methods for protein isoform determination have limitations in sensitivity, reproducibility, and capturing protein-level information.
  • Clinical proteomics faces computational challenges including low instrument sensitivity, high data noise, and variability.

Purpose of the Study:

  • To present a peptidomics method for identifying cancer-related and isoform-specific peptides for clinical proteomics.
  • To develop a large-scale screening approach for breast cancer-associated alternative splicing isoform markers.
  • To validate the identified markers through biological, statistical, and pathway analyses.

Main Methods:

  • Development of a Peptidomic Database of Human Protein Isoforms.
  • Implementation of a peptidomics approach for screening cancer-specific protein isoforms using LC-MS/MS.
  • Multi-faceted validation including biological, exon array, statistical, and pathway analyses.

Main Results:

  • Alternative splicing isoform markers were identified as independent indicators of breast cancer.
  • The peptidomics method effectively increased the number of identified alternative splicing isoform markers in clinical proteomics.
  • The study demonstrated the efficacy of the developed method in identifying cancer-specific protein isoform biomarkers.

Conclusions:

  • Alternative splicing isoform markers serve as independent biomarkers for breast cancer.
  • The presented peptidomics method is effective for identifying cancer-specific protein isoform biomarkers in clinical proteomics.
  • This approach enhances the discovery of alternative splicing isoform markers for improved cancer diagnostics.