Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

4.3K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.3K
What is Gene Expression?01:42

What is Gene Expression?

198.6K
Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
198.6K
What is Gene Expression?01:36

What is Gene Expression?

12.0K
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
12.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genomic distribution and context dependent functionality of novel WRKY transcription factor binding sites.

BMC genomics·2022
Same author

A dialogue-like cell communication mechanism is conserved in filamentous ascomycete fungi and mediates interspecies interactions.

Proceedings of the National Academy of Sciences of the United States of America·2022
Same author

Unusual DNA-binding properties of the Arabidopsis thaliana WRKY50 transcription factor at target gene promoters.

Plant cell reports·2020
Same author

A strong NF-κB p65 responsive cis-regulatory sequence from Arabidopsis thaliana interacts with WRKY40.

Plant cell reports·2019
Same author

Transcription factors involved in basal immunity in mammals and plants interact with the same MAMP-responsive cis-sequence from Arabidopsis thaliana.

Plant molecular biology·2018
Same author

From experiment-driven database analyses to database-driven experiments in Arabidopsis thaliana transcription factor research.

Plant science : an international journal of experimental plant biology·2017

Related Experiment Video

Updated: Mar 15, 2026

Analyzing Multifactorial RNA-Seq Experiments with DiCoExpress
05:22

Analyzing Multifactorial RNA-Seq Experiments with DiCoExpress

Published on: July 29, 2022

4.1K

In Silico Expression Analysis.

Julio Bolívar1, Reinhard Hehl1, Lorenz Bülow2

  • 1Institut für Genetik, Technische Universität Braunschweig, Spielmannstr. 7, 38106, Braunschweig, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|August 26, 2016
PubMed
Summary

Designing synthetic plant promoters requires understanding cis-sequences. This study introduces an in silico method to bioinformatically assess cis-sequence stress specificity, aiding in the design of functional, stress-responsive plant promoters.

Keywords:
Cis-elementsCis-regulatory sequencesExpression analysisIn silico validationPathoPlantPromoter sequences

More Related Videos

Using Human Differentially Expressed Gene Lists to Perform Downstream Pathway Enrichment Analysis and Target Prioritization
03:08

Using Human Differentially Expressed Gene Lists to Perform Downstream Pathway Enrichment Analysis and Target Prioritization

Published on: October 3, 2025

1.1K
Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets
06:40

Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets

Published on: February 23, 2024

1.8K

Related Experiment Videos

Last Updated: Mar 15, 2026

Analyzing Multifactorial RNA-Seq Experiments with DiCoExpress
05:22

Analyzing Multifactorial RNA-Seq Experiments with DiCoExpress

Published on: July 29, 2022

4.1K
Using Human Differentially Expressed Gene Lists to Perform Downstream Pathway Enrichment Analysis and Target Prioritization
03:08

Using Human Differentially Expressed Gene Lists to Perform Downstream Pathway Enrichment Analysis and Target Prioritization

Published on: October 3, 2025

1.1K
Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets
06:40

Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets

Published on: February 23, 2024

1.8K

Area of Science:

  • Plant molecular biology
  • Bioinformatics
  • Genomics

Background:

  • Cis-regulatory sequences control gene expression in plants.
  • Identifying stress-specific cis-sequences is crucial for designing functional synthetic promoters.
  • Experimental validation of candidate cis-sequences is time-consuming.

Purpose of the Study:

  • To demonstrate an in silico approach for validating the stress specificity of plant cis-regulatory sequences.
  • To present a method for predicting highly specific cis-regulatory elements for given plant stresses.
  • To introduce an online tool for bioinformatic assessment of cis-sequences.

Main Methods:

  • Correlation of genomic sequences with gene expression data.
  • In silico expression analysis to assess cis-sequence stress specificity.
  • Reverted in silico expression analysis for predicting cis-regulatory elements.

Main Results:

  • Successfully demonstrated in silico validation for a cold stress-responsive cis-regulatory sequence.
  • The described online tool can assess cis-sequences for various plant abiotic and biotic stresses.
  • The reverted analysis approach predicts potentially functional and highly specific cis-regulatory elements.

Conclusions:

  • In silico analysis provides an efficient alternative to experimental validation for assessing cis-sequence function.
  • The developed methods and tools facilitate the design of targeted synthetic plant promoters.
  • This approach supports the engineering of plants with enhanced stress resilience.