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Optimal Lentivirus Production and Cell Culture Conditions Necessary to Successfully Transduce Primary Human Bronchial Epithelial Cells
Published on: July 22, 2016
The establishment and characteristics of cell-adapted IBV strain H120
Yingshun Zhou1,2, Xin Yang1, Hongning Wang3
1School of Life Science, Key Laboratory of Animal Disease Prevention and Food Safety of Sichuan Province, Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, "985 Project" Science Innovative Platform for Resource and Environment Protection of Southwest, Sichuan University, Chengdu, 610064, China.
Abstract:
Avian infectious bronchitis virus is an important pathogen in poultry worldwide. Vaccination is the only effective way to prevent and control IBV infection. H120, one of the safest vaccine strains, which has been used worldwide as a primary vaccine, cannot adapt to passaged cells, which severely restricts the quality of the vaccine. Based on the reverse genetics of our previous research work, we constructed the recombinant R-H120-Beaudette-p65(S) strain by replacing the complete spike gene of H120 with the corresponding spike gene from the Beaudette p65 strain. Some biological characteristics, including replication kinetics, virulence and immunological properties of R-H120-Beaudette-p65(S) have been evaluated. The results showed that biological characteristics of R-H120-Beaudette-p65(S), such as replication kinetics in embryonated chicken eggs (ECEs) and embryo virulence, were similar to those of H120. In addition, R-H120-Beaudette-p65(S) could induce a similar antibody titre and provide up to 80 % immune protection in chickens challenged with the M41 strain. These results indicate that R-H120-Beaudette-p65(S) has the potential for further development as a cell-adapted vaccine.
