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A fast multispectral light synthesiser based on LEDs and a diffraction grating.

Gregor Belušič1, Marko Ilić1, Andrej Meglič1

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Area of Science:

  • Biomedical Optics
  • Spectroscopy
  • Optical Engineering

Background:

  • Optical experiments demand light sources with rapid switching, adjustable bandwidth, and intensity control.
  • Existing technologies may not meet the specific requirements for dynamic spectral analysis in biological systems.

Purpose of the Study:

  • To construct and validate a versatile, fast-switching wavelength combiner for optical applications.
  • To demonstrate its utility in biological research, including photoreceptor sensitivity measurements and hyperspectral imaging.

Main Methods:

  • A wavelength combiner was engineered using a reflective planar diffraction grating and light-emitting diodes (LEDs) emitting from 350 to 630 nm.
  • The device utilizes the first diffraction order of a reversed beam, launching the combined output into a fiber.
  • Pulse-width modulation drivers with 1 ms time resolution were employed for fast switching.

Main Results:

  • The combiner produced 22 spectral bands with approximately 15 nm spacing.
  • Validation included intracellular measurements of blowfly photoreceptor spectral sensitivity.
  • Hyperspectral imaging of Xenopus skin circulation demonstrated adequate resolution for hemoglobin absorption spectra.

Conclusions:

  • The developed wavelength combiner offers a robust, no-moving-parts solution for tunable light generation.
  • Its capabilities are suitable for diverse applications in visual physiology, biomedical optics, microscopy, and spectroscopy.
  • The device provides intrinsic multi-band output with low stray light.