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Rapid Antimicrobial Susceptibility Testing Using Forward Laser Light Scatter Technology.

Randall T Hayden1, Lani K Clinton2, Carolyn Hewitt3

  • 1St. Jude Children's Research Hospital, Department of Pathology, Memphis, Tennessee, USA randall.hayden@stjude.org.

Journal of Clinical Microbiology
|August 26, 2016
PubMed
Summary
This summary is machine-generated.

Forward laser light scatter (FLLS) offers a promising method for early antimicrobial susceptibility testing. This technique accurately determines bacterial susceptibility, potentially improving clinical decisions for bacterial infections.

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Area of Science:

  • Microbiology
  • Clinical Diagnostics
  • Biotechnology

Background:

  • Delayed antimicrobial susceptibility testing (AST) hinders timely clinical decisions for bacterial infections.
  • Current AST methods require significant incubation time, impacting treatment efficacy.
  • Need for rapid and accurate methods to guide antibiotic therapy.

Purpose of the Study:

  • To evaluate the utility of forward laser light scatter (FLLS) for early determination of antimicrobial susceptibility.
  • To assess the performance of the BacterioScan FLLS system against established commercial AST methods.

Main Methods:

  • Bacterial isolates of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa were tested.
  • Forward laser light scatter (FLLS) was employed using the BacterioScan system.
  • Comparison with two commercial systems: Vitek2 and MicroScan MIC panel.

Main Results:

  • The BacterioScan FLLS system demonstrated high categorical concordance with commercial AST methods.
  • Agreement with MicroScan was 88.9% (two minor errors).
  • Agreement with Vitek2 was 72.2% (five minor errors).

Conclusions:

  • Forward laser light scatter (FLLS) shows potential as a novel method for rapid antimicrobial susceptibility determination.
  • This technique could significantly reduce turnaround time for AST results.
  • FLLS offers a promising approach to enhance clinical decision-making in treating bacterial infections.