Development of a Cavity Disinfectant Containing Antibacterial Monomer MDPB

Area of Science:

• Dental Materials Science
• Microbiology
• Restorative Dentistry

Background:

• Dental cavity disinfectants are crucial for preventing secondary caries and ensuring restoration longevity.
• Existing disinfectants like chlorhexidine (CPS) may impact the bonding efficacy of resin cements.
• An experimental cavity disinfectant (ACC) containing 5% 12-methacryloyloxydodecylpyridinum bromide (MDPB) was developed.

Purpose of the Study:

• To evaluate the antibacterial effectiveness of ACC against common oral bacteria.
• To assess the influence of ACC on the dentin-bonding abilities of resin cements.
• To compare ACC's performance with a commercial chlorhexidine-based disinfectant (CPS).

Main Methods:

• Determined minimum inhibitory and bactericidal concentrations (MIC and MBC) for seven oral bacterial species.
• Evaluated antibacterial activity using agar disk diffusion tests and MIC/MBC assays for Streptococcus mutans.
• Assessed bactericidal effects in an S. mutans infected dentin model and measured shear bond strength of resin cements.

Main Results:

• Unpolymerized MDPB demonstrated potent antibacterial activity against all tested oral bacteria.
• ACC exhibited comparable inhibition zones to CPS, with similar MIC and MBC values for S. mutans.
• ACC showed significantly greater bactericidal effects in dentin tubules than CPS and did not negatively affect resin cement bond strength.

Conclusions:

• The experimental cavity disinfectant (ACC) containing 5% MDPB is a highly effective in vitro agent for eradicating dentinal bacteria.
• ACC demonstrates superior or comparable antibacterial efficacy to commercial chlorhexidine solutions.
• ACC does not adversely affect the bonding capabilities of self-adhesive or dual-cure resin cements to dentin.