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Does Formaldehyde Increase Cell Free DNA in Maternal Plasma Specimens?

Rintu R Jacob1, Renu Saxena2, Ishwar C Verma2

  • 1Centre of Medical Genetics, Sir Ganga Ram Hospital, New Delhi, India rintuj16@yahoo.co.in.

Laboratory Medicine
|August 26, 2016
PubMed
Summary

Formaldehyde treatment did not improve the recovery of cell-free fetal DNA (cffDNA) in maternal plasma. This study found no significant increase in the proportion of cffDNA with formaldehyde addition.

Keywords:
TaqMan probescell free fetal DNAcirculating cell free DNAformaldehydematernal plasmanoninvasive prenatal diagnosisreal time PCR

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Conflicting reports exist on formaldehyde's impact on cell-free fetal DNA (cffDNA) recovery from maternal plasma.
  • Formaldehyde is sometimes used to stabilize nucleic acids in biological samples.

Purpose of the Study:

  • To investigate the effect of formaldehyde treatment on the quantification of circulating cell-free DNA (cfDNA) in maternal plasma.

Main Methods:

  • Blood samples from 11 pregnant women carrying male fetuses were analyzed.
  • Real-time PCR assays (DYS14 and HBB) were used to quantify fetal and total cfDNA in formaldehyde-treated and untreated plasma.

Main Results:

  • Total cfDNA concentration was significantly reduced in formaldehyde-treated plasma compared to untreated plasma (P=.02).
  • The percentage of cffDNA did not differ significantly between formaldehyde-treated and untreated plasma samples (P=.15).

Conclusions:

  • Formaldehyde addition does not significantly enhance the proportion of cell-free fetal DNA in maternal plasma when samples are processed promptly.
  • The findings suggest formaldehyde may not be beneficial for cffDNA enrichment in routine prenatal diagnostics.