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Does Formaldehyde Increase Cell Free DNA in Maternal Plasma Specimens?
Rintu R Jacob1, Renu Saxena2, Ishwar C Verma2
1Centre of Medical Genetics, Sir Ganga Ram Hospital, New Delhi, India rintuj16@yahoo.co.in.
Background:
There have been conflicting observations reported in the literature regarding the effects of formaldehyde in the recovery of cell free fetal DNA (CFF DNA) from maternal plasma. The aim of the present study was to assess the effect of formaldehyde treatment on circulating cell free DNA.
Methods:
We conducted this study using blood specimens collected from 11 pregnant women, each of whom was carrying a male fetus. DYS14 and HBB real time assays were performed to quantify fetal and total circulating cell free DNA from formaldehyde treated and untreated maternal plasma specimens, respectively.
Results:
The concentration of total circulating cell free DNA in formaldehyde-treated maternal plasma was reduced, compared with untreated maternal plasma (n = 11; P = .02). The percentage of CFF DNA between formaldehyde-treated and untreated maternal plasma specimens did not differ significantly (n = 11; P = .15).
Conclusion:
Addition of formaldehyde does not significantly enhance the proportion of cell free fetal DNA when blood specimens are processed without delay.
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