Related Experiment Video
Updated: Mar 15, 2026
In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
Published on: March 29, 2019
An improved reprogrammable mouse model harbouring the reverse tetracycline-controlled transcriptional transactivator
S Alaei1, A S Knaupp1, S M Lim1
1Department of Anatomy and Developmental Biology, Monash University, Wellington Road, Clayton, VIC 3800, Australia; Australian Regenerative Medicine Institute, Monash University, Wellington Road, Clayton, VIC 3800, Australia; Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Wellington Road, Clayton, VIC 3800, Australia.
Researchers developed an improved mouse model for induced pluripotent stem cells (iPSCs) using a new transactivator system. This enhanced model significantly boosts reprogramming efficiency, enabling more detailed studies of iPSC generation.
Area of Science:
- Stem cell biology
- Epigenetics
- Molecular biology
Background:
- Reprogrammable mouse models expressing Oct-4, Klf-4, Sox-2, and c-Myc (OKSM) are crucial for studying induced pluripotent stem cells (iPSCs).
- Previous OKSM models utilized the m2 reverse tetracycline-controlled transactivator, leading to low reprogramming efficiency and limited intermediate cell isolation for analysis.
Purpose of the Study:
- To develop an improved OKSM mouse model with enhanced reprogramming efficiency.
- To facilitate mechanistic studies of the iPSC generation process by increasing the number of isolatable intermediate cells.
Main Methods:
- Engineering a novel OKSM mouse model utilizing the reverse tetracycline-controlled transactivator 3 (rtTA3).
- Comparing reprogramming efficiency and kinetics with previous m2 rtTA-based models.
Main Results:
- The new rtTA3-based OKSM model demonstrated over a 9-fold increase in reprogramming efficiency compared to the m2 rtTA model.
- The enhanced model yielded a greater number of reprogramming intermediate cells, crucial for downstream profiling.
- Reprogramming kinetics remained similar between the two model systems.
Conclusions:
- The improved OKSM mouse model using rtTA3 offers significantly higher reprogramming efficiency.
- This advancement will accelerate mechanistic investigations into the complex process of induced pluripotency.
- The model provides a more robust platform for dissecting the molecular events of cellular reprogramming.
