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Related Experiment Video

Updated: Mar 15, 2026

Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay

Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay

Published on: September 27, 2021

Flow-Enhanced Stability of Rolling Adhesion through E-Selectin.

Quhuan Li¹, Annica Wayman², Jiangguo Lin³

¹School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, China; Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, South China University of Technology, Guangzhou, China.

Biophysical Journal

|August 26, 2016

Summary

We developed an inverted flow chamber to study leukocyte rolling adhesion. Gravity affects cell attachment, and flow influences E-selectin-ligand bond dynamics, impacting rolling stability.

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Last Updated: Mar 15, 2026

Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay

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Published on: September 27, 2021

Analysis of Physiologic E-Selectin-Mediated Leukocyte Rolling on Microvascular Endothelium

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Systematic Analysis of In Vitro Cell Rolling Using a Multi-well Plate Microfluidic System

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Published on: October 16, 2013

Area of Science:

Biophysics
Cellular Mechanics
Immunology

Background:

Selectin-ligand interactions are crucial for leukocyte adhesion during inflammation.
Understanding the kinetic and mechanical factors governing leukocyte adhesion remains incomplete.
Gravity's influence on cell adhesion dynamics in flow systems is not well understood.

Purpose of the Study:

To design and utilize an inverted flow chamber for studying leukocyte-E-selectin interactions.
To investigate the effects of gravity and flow on cell adhesion and rolling stability.
To characterize the relationship between wall shear stress and the kinetics of E-selectin-ligand bonds.

Main Methods:

Development of a novel inverted flow chamber to compare cell adhesion in upright and inverted orientations.
Utilized HL-60 cells and E-selectin for adhesion assays.
Measured rolling adhesion lifetime and detachment dynamics under varying wall shear stress.

Main Results:

Gravity significantly influences cell tethering and reattachment, with distinct effects in upright versus inverted orientations.
Rolling adhesion lifetime exhibits a biphasic trend with wall shear stress, peaking at 0.4 dyn/cm(2).
The effective association on-rate (kon) and binding affinity (Ka) of E-selectin-ligand interactions are shear-dependent, with Ka also peaking at 0.4 dyn/cm(2).

Conclusions:

The inverted flow chamber enables precise measurement of rolling adhesion stability.
Flow-enhanced cell rolling stability is determined by the interplay between flow-dependent association and dissociation rates of E-selectin-ligand bonds.
Findings provide critical insights into the biophysical mechanisms of leukocyte adhesion in inflammatory contexts.