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Updated: Mar 15, 2026
On-Site Sampling and Extraction of Brain Tumors for Metabolomics and Lipidomics Analysis
Published on: May 31, 2020
Spectroscopic Characterization of Gliosarcomas-Do They Differ From Glioblastomas and Metastases?
Peter Raab1, Ulrich Pilatus, Elke Hattingen
1From the *Institute of Diagnostic and Interventional Neuroradiology, Hannover Medical School, Hannover; †Institute of Neuroradiology, Johann-Wolfgang-Goethe University, Frankfurt/Main; ‡Department of Neuroradiology, Clinic of Radiology, University Bonn, Bonn; §Department of Neurosurgery, Johann-Wolfgang-Goethe University, Frankfurt/Main; and ∥Department of Neurosurgery, Hannover Medical School, Hannover, Germany.
Objective:
To evaluate the spectroscopic pattern of gliosarcomas for differentiation from glioblastomas or metastases.
Methods:
H-nuclear magnetic resonance (NMR) spectroscopic intermediate echo time data of 5 patients with histologically proven gliosarcomas were compared with data of 17 metastases and 54 glioblastomas. Specialized H-NMR spectroscopy analysis software was used offline. Lipid and macromolecular resonances between 0.9 ppm and 1.4 ppm were compared besides the main metabolites using the Mann-Whitney U test.
Results:
Gliosarcomas showed higher lipid and macromolecule resonances and a higher lipid-choline ratio compared with glioblastomas (P < 0.024 and P < 0.036). Glioblastomas showed higher creatine concentrations compared with metastases (P < 0.007) but not compared with gliosarcomas. We found no significant differences between metastases and gliosarcomas.
Conclusions:
Gliosarcomas may mimic metastases on H NMR spectroscopy showing high signal intensities from lipid and macromolecule resonances. This tumor type should be suspected if conventional imaging suggests an intra-axial brain neoplasm in combination with high lipids in solid tumor parts.
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