Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

X-ray-induced mutations in cultured human thyroid cells.

N Nakamura1, R Sposto, R C Miller

  • 1Department of Radiobiology, Radiation Effects Research Foundation, Hiroshima, Japan.

Radiation Research
|July 1, 1989
PubMed
Summary

X-ray exposure increases mutation frequency in human thyroid cells. The mutation induction rate is similar to that observed in skin and mammary cells, confirming radiation

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Protein kinase C isozyme expression in human leukemia-lymphoma cell lines--an immunocytochemical study.

Hematological oncology·1992
Same author

A simple and rapid method for HLA-DQA1 genotyping by polymerase chain reaction-single strand conformation polymorphism and restriction enzyme cleavage analysis.

Electrophoresis·1992
Same author

[Pheochromocytoma discovered in a pregnant woman: a case report].

Hinyokika kiyo. Acta urologica Japonica·1992
Same author

The potentiating effect of KC-404 on prostacyclin-induced vasodilation in isolated human cerebral artery.

General pharmacology·1992
Same author

Effects of malnutrition on development of experimental pressure sores.

The Journal of dermatology·1992
Same author

[The pattern of neurological deterioration and the mechanism of neurological deficit in syringomyelia].

Rinsho shinkeigaku = Clinical neurology·1992

Area of Science:

  • Radiation biology
  • Molecular toxicology
  • Cellular mutagenesis

Background:

  • X-irradiation is a known mutagenic agent.
  • Understanding radiation-induced mutations is crucial for risk assessment.
  • Previous studies have investigated mutation rates in various human cell types.

Purpose of the Study:

  • To quantify the mutation induction rate in cultured human thyroid cells following X-irradiation.
  • To compare the radiation sensitivity of thyroid cells with other human epithelial cells.

Main Methods:

  • Cultured human thyroid cells were exposed to varying doses of X-rays (0, 1, and 2 Gy).
  • Cells were subsequently assayed for resistance to 6-thioguanine to determine mutant frequency.
  • Statistical analysis was performed to assess the dose-response relationship and calculate the mutation induction rate.

Related Experiment Videos

Main Results:

  • A statistically significant positive association was observed between X-ray dose and mutant frequency.
  • The average mutant frequency increased with increasing radiation dose.
  • The estimated mutation induction rate was 2.54 +/- 0.71 X 10(-5) per gray.

Conclusions:

  • X-irradiation induces mutations in human thyroid cells in a dose-dependent manner.
  • The mutation induction rate in thyroid cells is comparable to that of human skin fibroblasts and mammary epithelial cells.
  • These findings support the similarity of radiation-induced mutation processes across different human epithelial cell types.