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Hydrogen sulfide protects against TNF-α induced neuronal cell apoptosis through miR-485-5p/TRADD signaling

Zhiqiang Chen1, Zhonghe Zhang2, Dongdong Zhang3

  • 1Department of Orthopedic Surgery, Liaocheng People's Hospital, 67 Dongchang West Road, Liaocheng 252000, China.

Biochemical and Biophysical Research Communications
|August 27, 2016
PubMed
Abstract

Insights

Hydrogen sulfide (H2S) protects neural cells from injury by regulating miR-485-5p and TRADD. This study reveals the miR-485-5p/TRADD axis involved in H2S-mediated neuroprotection against TNF-α-induced apoptosis.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Biochemistry

Background:

  • MicroRNAs (miRNAs) regulate pathological changes in humans.
  • miR-485-5p is affected by spinal cord ischemia/reperfusion (I/R) injury.
  • Hydrogen sulfide (H2S) shows neuroprotective potential.

Purpose of the Study:

  • Investigate the role of miR-485-5p in H2S-mediated neuroprotection.
  • Elucidate the mechanism of H2S in protecting neural cells from injury.
  • Determine the involvement of the miR-485-5p/TRADD axis in H2S neuroprotection.

Main Methods:

  • Detected serum TNF-α and miR-485-5p in spinal cord I/R patients and controls.
  • Administered H2S using GYY4137 treatment in vitro.
  • Utilized quantitative RT-PCR, western blot, and luciferase reporter assays to analyze gene expression and protein interactions.

Main Results:

  • Serum TNF-α was reduced in I/R patients.
  • H2S (GYY4137) reversed TNF-α-induced neuronal apoptosis in vitro.
  • H2S protected neuronal cells via a TRADD-dependent pathway.
  • miR-485-5p was confirmed to regulate TRADD expression.
  • The miR-485-5p/TRADD axis was involved in H2S neuroprotection.

Conclusions:

  • Identified the miR-485-5p/TRADD axis in H2S neuroprotection.
  • Demonstrated H2S protects against TNF-α-induced neuronal apoptosis.
  • Established the mechanism of H2S neuroprotection involving miR-485-5p and TRADD.
Keywords:
AGE1.HNHydrogen sulfideSY-SH-5YTRADDmiR-485-5p

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