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Updated: Mar 15, 2026

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Author Spotlight: Unraveling the Interplay Between Trichoderma stromaticum and the Mammalian Immune System
Published on: October 20, 2023
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Isolation and Mass Production of Trichoderma
Artemio Mendoza-Mendoza1, Annabel Clouston2, Jin-Hua Li2
1Bio-Protection Research Centre, Lincoln University, 85084, Lincoln, 7647, New Zealand. artemio.mendoza@lincoln.ac.nz.
Methods in Molecular Biology (Clifton, N.J.)
|August 28, 2016
Summary
This chapter details common methods for isolating, identifying, and propagating Trichoderma strains, which are widely used as fungal biocontrol agents for plant diseases.
Area of Science:
- Agricultural Science
- Mycology
- Plant Pathology
Background:
- Trichoderma species are extensively utilized as commercial fungal biocontrol agents.
- Significant research exists on their biocontrol potential and underlying mechanisms.
Purpose of the Study:
- To summarize common methodologies for Trichoderma strain analysis.
- To provide guidance on isolation, identification, and mass propagation techniques.
Main Methods:
- Isolation of Trichoderma strains from various sources.
- Identification using morphological and molecular techniques.
- Mass propagation protocols for large-scale application.
Main Results:
- Detailed overview of established protocols for Trichoderma handling.
- Emphasis on practical methods for biocontrol program implementation.
Conclusions:
- Standardized methods are crucial for effective utilization of Trichoderma biocontrol agents.
- This chapter serves as a practical guide for researchers and practitioners.
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