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Updated: Mar 15, 2026
The MultiBac Protein Complex Production Platform at the EMBL
Published on: July 11, 2013
Rapid evolution of hyaluronan synthase to improve hyaluronan production and molecular mass in Bacillus subtilis
Linpei Zhang1, Hao Huang1, Hao Wang1
1The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Objectives:
To improve the production and molecular mass of the glycosaminoglycan hyaluronan (HA) in Bacillus subtilis by engineering hyaluronan synthase (HAS) from Streptococcus zooepidemicus.
Results:
By mutating regions within HAS intracellular domains, five positive variants exhibiting higher HA production (from 1.22 to 2.24 g l-1) and molecular mass values (from 1.20 to 1.36 × 106 Da) were constructed and characterized. Overexpression of the V5 variant and the genes tuaD and glmU increased HA production and molecular mass to 2.8 g l-1 and 2.4 × 106 Da, respectively.
Conclusions:
This study provides a novel strategy for improving HA production and its molecular mass.
