Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

66.2K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
66.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dulaglutide versus empagliflozin as add-on therapy to metformin and sulfonylurea in type 2 diabetes: a randomized pilot study with exploratory metabolomic and microbiome analyses.

Frontiers in endocrinology·2026
Same author

Reflecting Local Economic Parameters in ALARA Evaluations for the Reuse of Decommissioning Remaining Building.

Journal of radiological protection : official journal of the Society for Radiological Protection·2026
Same author

A fully integrated sample-to-answer molecular diagnostic platform for rapid identification of four major <i>Aspergillus</i> species.

Frontiers in microbiology·2026
Same author

Self-Assembly of Hierarchical Macroporous Metal-Organic Framework Films Guided by Hydrophilic-Lipophilic Balance.

Journal of the American Chemical Society·2026
Same author

Effects of illite or bentonite on cytotoxicity, antibacterial and adsorption capacity in porcine intestinal epithelial cells.

Journal of animal science and technology·2026
Same author

IFI35 suppresses the transcription of hepatitis B virus cccDNA minichromosome via promoting HNF4α proteasomal degradation.

Journal of biomedical science·2026

Related Experiment Video

Updated: Mar 15, 2026

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
08:23

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

14.1K

Microparticle-based RT-qPCR for highly selective rare mutation detection.

Eun Hae Oh1, Seungwon Jung1, Won Jin Kim2

  • 1Center for BioMicrosystems, Brain Science Institute, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea.

Biosensors & Bioelectronics
|August 28, 2016
PubMed
Summary
This summary is machine-generated.

This study introduces a novel RT-qPCR protocol using primer-immobilized networks (PINs) for highly specific RNA detection. This method integrates capture, reverse transcription, and amplification in a single particle, significantly reducing non-specific signals for disease biomarkers.

Keywords:
Bcr-Abl fusion transcriptPrimer-immobilized particlesRT-qPCR

More Related Videos

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations
10:41

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations

Published on: March 29, 2017

12.4K
Author Spotlight: Advancing the Detection of Low-Frequency Mutations in Cancer Tissues
07:17

Author Spotlight: Advancing the Detection of Low-Frequency Mutations in Cancer Tissues

Published on: August 23, 2024

1.9K

Related Experiment Videos

Last Updated: Mar 15, 2026

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
08:23

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

14.1K
Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations
10:41

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations

Published on: March 29, 2017

12.4K
Author Spotlight: Advancing the Detection of Low-Frequency Mutations in Cancer Tissues
07:17

Author Spotlight: Advancing the Detection of Low-Frequency Mutations in Cancer Tissues

Published on: August 23, 2024

1.9K

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Medical Diagnostics

Background:

  • Quantitative reverse transcription polymerase chain reaction (RT-qPCR) is a key technique for detecting disease-specific RNAs.
  • Traditional RT-qPCR involves separate reverse transcription (RT) and quantitative polymerase chain reaction (qPCR) steps, risking sample loss and non-specific amplification.

Purpose of the Study:

  • To develop a novel, integrated RT-qPCR protocol for enhanced specificity and sensitivity in RNA detection.
  • To minimize cDNA loss and suppress non-specific amplification in complex biological samples.

Main Methods:

  • A new RT-qPCR protocol utilizing primer-immobilized networks (PINs) within a single particle was developed.
  • The protocol integrates RNA capture, reverse transcription (RT), and amplification (qPCR) sequentially within the PIN particle.
  • Reaction solutions were exchanged to perform RT and amplification without loss of intermediate cDNA products.

Main Results:

  • The primer-immobilized networks (PINs) protocol dramatically suppressed the production of undesired cDNAs through specific target RNA capture.
  • Highly sensitive detection of the Bcr-Abl fusion transcript, a biomarker for chronic myeloid leukemia, was achieved.
  • The method demonstrated excellent restraint of non-specific signals, detecting a single mutant leukemic cell among 10^4 normal cells.

Conclusions:

  • The novel particle-based RT-qPCR protocol offers a streamlined and highly specific method for target RNA detection.
  • This integrated approach is advantageous for analyzing complex samples and detecting low-abundance disease biomarkers with high accuracy.