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Related Concept Videos

Bacterial Transformation01:33

Bacterial Transformation

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In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
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Transformation01:26

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Microbial communities are dynamic environments where cell lysis releases free DNA into the surroundings. Other cells can take up this extracellular DNA through a process known as transformation.When a cell incorporates this foreign DNA into its genome, resulting in genetic modification, the process is known as transformation. Cells capable of this process are termed competent. Competence can be natural, as observed in certain bacteria and archaea, or artificially induced in the...
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Efficient transformation of Staphylococcus aureus using multi-pulse electroporation.

Junzo Hisatsune1, Yusuke Sato'o1, Liansheng Yu1

  • 1Department of Bacteriology, Hiroshima University Graduate School of Biomedical & Health Sciences, Hiroshima, Japan; Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, Japan.

Journal of Microbiological Methods
|August 29, 2016
PubMed
Summary

A novel multi-pulse electroporation method significantly enhances Staphylococcus aureus transformation efficiency. This new system achieves over 3.9×10^5 transformed cells per microgram of plasmid DNA in a single electroporation.

Keywords:
CompetencyELEPO21Multi-pulse electroporationPlasmid DNAPoring pulseStaphylococcus aureusTransfer pulseTransformation

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Area of Science:

  • Microbiology
  • Biotechnology
  • Molecular Biology

Background:

  • Staphylococcus aureus is a significant human pathogen.
  • Efficient genetic transformation is crucial for studying and manipulating S. aureus.
  • Conventional electroporation methods can be inefficient for certain bacterial species.

Purpose of the Study:

  • To evaluate a new multi-pulse electroporation system for transforming Staphylococcus aureus.
  • To compare the transformation efficiency of the new system with conventional electroporation.
  • To optimize electroporation parameters for enhanced S. aureus transformation.

Main Methods:

  • Development and application of a novel multi-pulse electroporation system.
  • Transformation of Staphylococcus aureus RN4220 using the new system.
  • Comparison of transformation efficiency with a conventional electroporation system.
  • Optimization of poring and transfer pulse parameters.

Main Results:

  • The multi-pulse electroporation system achieved high transformation efficiency.
  • Yielded over 3.9×10^5 S. aureus RN4220 transformed cells per 1μg plasmid DNA.
  • Demonstrated superior performance compared to conventional electroporation methods.

Conclusions:

  • The new multi-pulse electroporation system offers a highly efficient method for transforming Staphylococcus aureus.
  • This technology facilitates genetic manipulation and research of S. aureus.
  • Optimized pulse parameters are key to achieving high transformation yields.