Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A simple, automated functional assay for protein C.

P A Walker1, K A Bauer, J McDonagh

  • 1Department of Pathology, Beth Israel Hospital, Boston, Massachusetts 02215.

American Journal of Clinical Pathology
|August 1, 1989
PubMed
Summary

A new automated assay using copperhead venom can accurately measure functional protein C levels. This simple method is crucial for clinical labs to detect thrombotic disease risk associated with low protein C.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enhancements in Laser-Direct-Drive Nuclear Performance with Target Radius.

Physical review letters·2026
Same author

Active energy compression of a laser-plasma electron beam.

Nature·2025
Same author

Applications of a Rayleigh-Taylor model to direct-drive laser fusion.

Physical review. E·2024
Same author

Persistent Hot-Spot Mix in Cryogenic Direct-Drive Fusion Experiments.

Physical review letters·2024
Same author

Optical characterization of the on-target OMEGA focal spot at high energy using the full-beam in-tank diagnostic.

Applied optics·2020
Same author

Tripled yield in direct-drive laser fusion through statistical modelling.

Nature·2019

Area of Science:

  • Biochemistry
  • Hematology
  • Clinical Diagnostics

Background:

  • Protein C is a vital anticoagulant, regulating blood clotting.
  • Imbalances in coagulation pathways, including low protein C, increase thrombosis risk.
  • Accurate measurement of functional protein C is essential for clinical laboratories.

Purpose of the Study:

  • To develop and validate a simple, automated functional assay for protein C.
  • To provide a reliable method for clinical coagulation testing.

Main Methods:

  • An automated functional protein C assay was developed using copperhead venom for activation.
  • The assay was implemented on a centrifugal analyzer (COBAS Bio).
  • Assay specificity was confirmed by comparing results with radioimmunoassay.

Main Results:

  • The automated assay provides a simple and effective method for measuring functional protein C.
  • The assay demonstrated specificity when compared to a radioimmunoassay.

Conclusions:

  • The described automated assay is suitable for clinical laboratories.
  • This assay can aid in identifying patients at risk for thrombotic diseases due to low protein C levels.

Related Experiment Videos