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Gene expression profile analysis of dbpA knockdown in colorectal cancer cells
Ruiting Liu1, Guorong Wang1, Chang Liu2
1Department of General Surgery, Shaanxi Provincial People's Hospital, The Third Affiliated Hospital, Medical College, Xi'an Jiao Tong University, Xi'an, 710068, China.
Abstract:
DNA-binding protein A (dbpA) has been reported associated with the pathogenesis and development of various cancers. However, no evidence showed the gene expression profiling alternation involved in dbpA knockdown in colorectal cancer (CRC) cells. Small interference RNA (siRNA) method was used to knock down dbpA expression in SW620 cells. The changes of gene expression profiles in dbpA knockdown SW620 cells were determined by microarray analysis and Western blot. A total of 578 genes expressed differentially (twofold change), 181 genes were up-regulated, and 397 down-regulated in the dbpA knockdown group in comparison with the control group. The discrimination reliability was further verified by principal component analysis and Pearson correlation. Gene ontology (GO) pathway analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that the most significantly expressed genes were linked to MAPK signaling pathway. Furthermore, Western blot verified that dbpA knockdown directly inhibited the activations of TAK1, p38, and JNK in CRC cells. In conclusion, dbpA knockdown in SW620 cells altered the expression of carcinogenesis-associated genes in CRC and the involvement of dbpA on CRC might through MAPK signaling pathway, which might provide valuable evidence to further investigate the correlation between dbpA expression and CRC development.
Insights
DNA-binding protein A (dbpA) knockdown in colorectal cancer (CRC) cells alters gene expression. This suggests dbpA influences CRC development via the MAPK signaling pathway.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- DNA-binding protein A (dbpA) is implicated in cancer pathogenesis.
- Limited data exists on dbpA's role in colorectal cancer (CRC) gene expression.
- Understanding dbpA's molecular mechanisms in CRC is crucial.
Purpose of the Study:
- To investigate the impact of dbpA knockdown on gene expression profiles in CRC cells.
- To identify specific signaling pathways affected by dbpA modulation in CRC.
- To provide molecular insights into dbpA's role in CRC development.
Main Methods:
- Small interference RNA (siRNA) was used to knockdown dbpA expression in SW620 CRC cells.
- Microarray analysis and Western blot were employed to assess gene expression changes.
- Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed.
Main Results:
- dbpA knockdown resulted in 578 differentially expressed genes (181 up-regulated, 397 down-regulated).
- Pathway analysis highlighted the MAPK signaling pathway as significantly affected.
- Western blot confirmed that dbpA knockdown inhibited TAK1, p38, and JNK activation.
Conclusions:
- dbpA knockdown alters the expression of carcinogenesis-associated genes in CRC.
- dbpA appears to influence CRC progression through the MAPK signaling pathway.
- These findings offer valuable evidence for further research on dbpA and CRC development.
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