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Mosquito-Associated Virus Isolation from Field-Collected Mosquitoes
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A Multicomponent Animal Virus Isolated from Mosquitoes.

Jason T Ladner1, Michael R Wiley1, Brett Beitzel1

  • 1Center for Genome Sciences, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA.

Cell Host & Microbe
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Summary

This study identifies Guaico Culex virus (GCXV) as a multicomponent RNA virus in mosquitoes, expanding knowledge of segmented animal viruses. Findings suggest these viruses are relevant to animal and human health.

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Area of Science:

  • Virology
  • Molecular Biology
  • Genomics

Background:

  • RNA viruses utilize diverse genome organization strategies, including multicomponent genomes where each segment is packaged separately.
  • Multicomponent genomes are prevalent in plant and fungal viruses, but their occurrence in animal viruses is less understood.
  • The Jingmenvirus clade, related to Flaviviridae, includes segmented viruses, but their genome composition is not fully characterized.

Purpose of the Study:

  • To characterize a novel multicomponent RNA virus, Guaico Culex virus (GCXV), isolated from mosquitoes.
  • To investigate the genome organization and replication requirements of GCXV.
  • To expand the known host range and understanding of Jingmenvirus diversity.

Main Methods:

  • Isolation and characterization of Guaico Culex virus (GCXV) from mosquito samples.
  • Genome sequencing and analysis of viral RNA segments.
  • Comparative analysis with related segmented viruses, including Jingmen tick virus.

Main Results:

  • GCXV possesses a five-segment RNA genome, with each segment appearing to be separately packaged.
  • The smallest GCXV genomic segment is dispensable for replication and shows variable presence in natural infections.
  • A variant of Jingmen tick virus was identified in a Ugandan red colobus monkey, extending the host range of Jingmenviruses.

Conclusions:

  • This research provides evidence for the existence of multicomponent RNA viruses in animal hosts.
  • The findings highlight the potential significance of multicomponent animal viruses for both animal and human health.
  • The study contributes to understanding the diversity and evolution of segmented RNA viruses.