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Cloning identification and functional analysis of human IL-17A promoter
Ming-Jie Hu1, Shou-Wei Wu1, Mei-Li Wei1
1School of Life Science, Institute of Neurobiology, Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, Bengbu, Anhui, 233030, China.
Objective:
To conduct the cloning identification and characterization of the sequence of human IL-17A promoter so as to analyze the regulatory mechanism of the gene expression of IL-17.
Methods:
First of all, the potential promoter region of IL-17A was found by means of the bioinformatics methods. Then, it was cloned into the reporter vector with PCR technique. Finally, the activity of the test promoter was determined by dual luciferase reporter system.
Results:
Two transcriptional start points of the upper region, 600 bp and 1000 bp, of IL-17A were obtained by PCR clone and proved to have certain activities by dual luciferase reporter system. Also, they could be activated by IL-17A activator STAT3, which could start the expression of the reported gene.
Conclusions:
Clone established the regulatory region of human IL-17A promoter, which provided bases to the subsequent function research.
