Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

3.0K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
3.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hunting ecology predicts eye arrangements in the modular visual system of spiders.

Current biology : CB·2026
Same author

3D virtual histopathology of human breast tissues using an X-ray grating interferometry micro CT system.

Experimental and molecular pathology·2026
Same author

Histology-guided 3D virtual staining of microCT-imaged lung tissue via deep learning.

Journal of the Royal Society, Interface·2026
Same author

Degeneration dependent changes in human knee cartilage mechanical properties revealed by synchrotron tomography based finite element modeling.

Osteoarthritis and cartilage open·2026
Same author

Correction: Correlative X-ray Imaging to Reveal the Dissolution of Nanoparticles and Nutrient Transport in Plant Foliar Fertilization.

Frontiers in plant science·2026
Same author

Synchrotron phase-contrast microtomography reveals the impact of degeneration on the 3D structure of human articular cartilage.

Osteoarthritis and cartilage·2026

Related Experiment Video

Updated: Mar 15, 2026

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
08:41

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

12.1K

A multi-purpose imaging endstation for high-resolution micrometer-scaled sub-second tomography.

Goran Lovric1, Rajmund Mokso2, Christian M Schlepütz3

  • 1Swiss Light Source, Paul Scherrer Institute, 5232 Villigen, Switzerland; Centre d'Imagerie BioMédicale, École Polytechnique Fédérale de Lausanne, Lausanne 1015, Switzerland.

Physica Medica : PM : an International Journal Devoted to the Applications of Physics to Medicine and Biology : Official Journal of the Italian Association of Biomedical Physics (AIFB)
|August 30, 2016
PubMed
Summary

High-speed X-ray imaging enables detailed in vivo lung studies. Ultra-short exposures and precise triggering are key for micrometer-scale tomographic microscopy with reduced radiation dose.

Keywords:
In vivoTime-resolved tomography 2010X-ray imaging

More Related Videos

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
08:51

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

Published on: May 27, 2008

13.7K
High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT
08:57

High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT

Published on: June 21, 2011

19.3K

Related Experiment Videos

Last Updated: Mar 15, 2026

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
08:41

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

12.1K
Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
08:51

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

Published on: May 27, 2008

13.7K
High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT
08:57

High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT

Published on: June 21, 2011

19.3K

Area of Science:

  • Advanced imaging techniques
  • Synchrotron-based X-ray microscopy
  • Dynamic process visualization

Background:

  • Dynamic processes require flexible endstations for high-speed control.
  • Previous work demonstrated 20Hz in situ tomography and in vivo lung imaging feasibility.

Purpose of the Study:

  • To present developments in instrumentation for high-speed, time-resolved imaging.
  • To detail technical requirements for in vivo tomographic lung microscopy.
  • To optimize acquisition schemes for dose reduction and image quality.

Main Methods:

  • Development of a flexible endstation for complex component control.
  • Implementation of ultra-short single-projection exposures.
  • Integration of accurate triggering capabilities for dynamic events.

Main Results:

  • Achieved in situ tomographic measurements at 20Hz.
  • Demonstrated feasibility of in vivo lung imaging at micrometer resolution.
  • Identified optimal acquisition strategies for high-quality, low-dose in vivo microscopy.

Conclusions:

  • Ultra-short exposures and precise triggering are crucial for in vivo lung imaging.
  • The developed endstation supports high-speed imaging for diverse time-resolved studies.
  • This technology advances the study of dynamic biological and material processes.