Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Size and Structure of Viral Genomes01:26

Size and Structure of Viral Genomes

993
Viral genomes exhibit remarkable diversity in size, structure, and composition, influencing their replication strategies and interactions with host cells. These genomes consist of either DNA or RNA and may be linear or circular. Additionally, they can be single-stranded or double-stranded, with each configuration affecting how the virus propagates within a host. RNA viruses, for instance, generally have smaller genomes than DNA viruses, a factor that contributes to their high mutation rates and...
993
Viruses with RNA Genomes01:29

Viruses with RNA Genomes

1.2K
RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
1.2K
Retroviruses02:33

Retroviruses

15.6K
Retroviruses and retrotransposons both insert copies of their genetic elements into the genome of the host cell. Thus, the viral genes are passed on when the host genome is replicated or translated. A typical retroviral DNA sequence contains 3-4 genes that encode the different proteins required for its structural assembly and function as a molecular parasite. This DNA is transcribed into a single mRNA, which is very similar in structure to conventional mRNAs, i.e., it is capped at the 5’...
15.6K
Retrovirus Life Cycles01:10

Retrovirus Life Cycles

50.3K
Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the...
50.3K
Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

6.5K
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
6.5K
Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

5.9K
5.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Flexible artificial lateral line based on luminous flux for underwater velocity vector estimation.

National science review·2026
Same author

Retrospective non-target screening reveals the occurrence and environmental risk of pharmaceutical transformation products in the Pearl River Basin.

Environment international·2026
Same author

Cell crowding initiates tumor invasion by triggering a nanoscale topography transition of plasma membranes.

Acta biomaterialia·2026
Same author

"Double-hit" precipitates fulminant cardiac dysfunction in a child with homozygous CAP2 variant: a case report.

Frontiers in cardiovascular medicine·2026
Same author

Development and validation of a nomogram for estimating frailty probability in patients on maintenance hemodialysis.

Scientific reports·2026
Same author

Adhesin gene overexpression stimulates biofilm formation and catalytic performance in recombinant Escherichia coli.

Bioresource technology·2026

Related Experiment Video

Updated: Mar 15, 2026

Identifying Dysregulated Genes Induced by Kaposi's Sarcoma-associated Herpesvirus KSHV
07:02

Identifying Dysregulated Genes Induced by Kaposi's Sarcoma-associated Herpesvirus KSHV

Published on: September 14, 2010

13.1K

Kaposi's Sarcoma Herpesvirus Genome Persistence.

Franceline Juillard1, Min Tan1, Shijun Li1

  • 1Departments of Medicine, Brigham and Women's Hospital, Harvard Medical School Boston, MA, USA.

Frontiers in Microbiology
|August 30, 2016
PubMed
Summary

Kaposi's sarcoma-associated herpesvirus (KSHV) causes lifelong infections, often in immunocompromised individuals. The latency-associated nuclear antigen (LANA) protein is crucial for KSHV genome persistence during latent infection.

Keywords:
DNA bindingKSHVchromosomelatency-associated nuclear antigenviral persistence

More Related Videos

Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining
13:22

Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining

Published on: January 23, 2014

18.8K
Quantitative Fluorescence In Situ Hybridization FISH and Immunofluorescence IF of Specific Gene Products in KSHV-Infected Cells
06:21

Quantitative Fluorescence In Situ Hybridization FISH and Immunofluorescence IF of Specific Gene Products in KSHV-Infected Cells

Published on: August 27, 2019

13.3K

Related Experiment Videos

Last Updated: Mar 15, 2026

Identifying Dysregulated Genes Induced by Kaposi's Sarcoma-associated Herpesvirus KSHV
07:02

Identifying Dysregulated Genes Induced by Kaposi's Sarcoma-associated Herpesvirus KSHV

Published on: September 14, 2010

13.1K
Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining
13:22

Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining

Published on: January 23, 2014

18.8K
Quantitative Fluorescence In Situ Hybridization FISH and Immunofluorescence IF of Specific Gene Products in KSHV-Infected Cells
06:21

Quantitative Fluorescence In Situ Hybridization FISH and Immunofluorescence IF of Specific Gene Products in KSHV-Infected Cells

Published on: August 27, 2019

13.3K

Area of Science:

  • Virology
  • Oncology
  • Molecular Biology

Background:

  • Kaposi's sarcoma-associated herpesvirus (KSHV) is linked to Kaposi's sarcoma, primary effusion lymphoma, and multicentric Castleman's disease.
  • These KSHV-associated diseases predominantly affect immunocompromised individuals, particularly those with AIDS.
  • KSHV establishes lifelong infections characterized by a predominantly latent phase in tumor cells.

Purpose of the Study:

  • To investigate the role of the latency-associated nuclear antigen (LANA) in KSHV genome persistence.
  • To understand the molecular mechanisms by which LANA ensures viral genome replication and segregation.
  • To identify LANA as a potential therapeutic target for KSHV infection and associated diseases.

Main Methods:

  • Analysis of KSHV latency and gene expression during infection.
  • Characterization of LANA protein domains and their functions in viral genome maintenance.
  • Investigation of LANA's interaction with host cell mitotic chromosomes and viral DNA.

Main Results:

  • LANA is a key KSHV-expressed gene during latency, essential for viral genome persistence.
  • LANA mediates viral DNA replication and ensures accurate segregation of episomes to daughter nuclei.
  • N-terminal LANA binds histones on mitotic chromosomes, while C-terminal LANA binds viral DNA, facilitating genome tethering.

Conclusions:

  • LANA plays a critical role in maintaining the KSHV episome during cell division.
  • The molecular interactions mediated by LANA are essential for lifelong KSHV persistence.
  • Targeting LANA offers a promising strategy for therapeutic intervention against KSHV-driven diseases.