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An Easy Method for Plant Polysome Profiling
Published on: August 28, 2016
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Polysome Preparation, RNA Isolation and Analysis
1Department of Pediatrics, Emory University, Atlanta, USA.
Bio-Protocol
|August 30, 2016
Abstract:
During mRNA translation, 40S and 60S ribosomal subunits bind to target mRNA forming into an 80S complex (monosome). This ribosome moves along the mRNA during translational elongation to facilitate tRNA reading codon, where translation is activated and many monosome can bind the same mRNA simutaneously, which forms polysomes. Polysomes can be size-fractionated by sucrose density gradient centrifugation. The more specific mRNA in polysomes implies more active translational status of the mRNA.
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