Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Multimodal characterization of variation in neuronal types in the mouse basal ganglia.

bioRxiv : the preprint server for biology·2026

Same author

Connecting single-cell transcriptomes to projectomes in the mouse visual cortex.

Nature·2026

Same author

Conserved Cell Type Signatures Across the Brainstem and Spinal Cord in the Mouse Central Nervous System.

bioRxiv : the preprint server for biology·2026

Same author

Brain-wide topographic coordination of rotating waves.

Science (New York, N.Y.)·2026

Same author

Developmentally dynamic, non-convergent transcriptomic profiles in CNV models for schizophrenia risk.

Molecular psychiatry·2026

Same author

Search, organize, aggregate and share image data with BioFile Finder (BFF).

Nature methods·2026

Same journal

Neural timescales from a computational perspective.

Nature neuroscience·2026

Same journal

Author Correction: Spinal cord Tau pathology induces tactile deficits and cognitive impairment in Alzheimer's disease via dysregulation of CCK neurons.

Nature neuroscience·2026

Same journal

Hippocampal theta sweeps indicate goal direction during navigation.

Nature neuroscience·2026

Same journal

Just how goal-directed are hippocampal theta sweeps, anyway?

Nature neuroscience·2026

Same journal

Goal-directed hippocampal theta sweeps during memory-guided navigation.

Nature neuroscience·2026

Same journal

Connectomic evidence that ordered activity drives neuromuscular network formation.

Nature neuroscience·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 15, 2026

Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double In Vitro Transcription with Absolute Counts Sequencing DIVA-Seq

Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double In Vitro Transcription with Absolute Counts Sequencing DIVA-Seq

Published on: October 26, 2018

Disentangling neural cell diversity using single-cell transcriptomics.

Jean-Francois Poulin¹, Bosiljka Tasic², Jens Hjerling-Leffler³

¹Department of Neurology, Northwestern University, Chicago, Illinois, USA.

Nature Neuroscience

|August 30, 2016

Summary

This summary is machine-generated.

This study guides the classification of mammalian neural cell types using advanced single-cell gene expression profiling. It details methods for categorizing neurons based on molecular properties to understand nervous system complexity.

More Related Videos

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Published on: May 22, 2017

Related Experiment Videos

Last Updated: Mar 15, 2026

Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double In Vitro Transcription with Absolute Counts Sequencing DIVA-Seq

Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double In Vitro Transcription with Absolute Counts Sequencing DIVA-Seq

Published on: October 26, 2018

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Published on: May 22, 2017

Area of Science:

Neuroscience
Genomics
Cell Biology

Background:

Mammalian nervous systems exhibit extensive cellular specialization with diverse neuron types.
Understanding neuronal heterogeneity is crucial for deconstructing nervous system function.
Previous classification methods were limited in scope and resolution.

Purpose of the Study:

To provide a guide for classifying mammalian neural cell types.
To leverage single-cell gene expression profiling for neuronal categorization.
To address the challenge of disentangling neuronal heterogeneity.

Main Methods:

Utilizing high-throughput single-cell RNA sequencing.
Employing multiplexed quantitative RT-PCR.
Developing a conceptual and practical framework for classification.

Main Results:

Demonstrated the feasibility of systematic neuronal classification.
Enabled categorization of individual neurons into molecularly defined groups.
Provided a scalable approach to map neuronal diversity.

Conclusions:

Single-cell gene expression profiling is a powerful tool for neural cell type classification.
This approach facilitates a deeper understanding of nervous system organization.
The guide offers a practical pathway for future neuroscience research.