Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Calcium tartrate gel.

A A Akhrem1, A P Drozhdenyuk

  • 1Institute of Bioorganic Chemistry, Byelorussian SSR Academy of Sciences, Minsk, USSR.

Analytical Biochemistry
|May 15, 1989
PubMed
Summary
This summary is machine-generated.

A novel calcium tartrate gel adsorbent was developed for chromatography. This adsorbent demonstrates comparable binding capacity to hydroxyapatite for biomolecules like BSA, RNA, and DNA.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Spatial structure of the rp142 peptide containing the immunodominant epitope of the HIV-1 gp120 protein. A theoretical study].

Biofizika·1999
Same author

Melting of cross-linked DNA. III. Calculation of differential melting curves.

Journal of biomolecular structure & dynamics·1998
Same author

[Three-dimensional molecular model of the immunodominant epitope of GP 120 protein of HIV-1 virus].

Doklady Akademii nauk·1998
Same author

Melting of cross-linked DNA: II. Influence of interstrand linking on DNA stability.

Journal of biomolecular structure & dynamics·1997
Same author

[Theoretical study of the spatial structure of the Ala-Ser-Thr-Thr-Thr-Asn-Tyr-Thr segment of the HIV gp120 protein, responsible for binding of the virus with the T-cell T4 receptor].

Molekuliarnaia biologiia·1997
Same author

[Theoretical conformational NMR analysis of peptide RP342 of the immunogenic fragment of HIV-1 protein gp120].

Doklady Akademii nauk·1996
Same journal

Lysozyme assay using a rationally designed GN4G2 substrate with coupled β-glucosidase reaction.

Analytical biochemistry·2026
Same journal

The long run: A tribute to Arthur Joseph Lawrence Cooper.

Analytical biochemistry·2026
Same journal

Evaluation of a method for affinity measurement using solution equilibrium titration with magnetic beads.

Analytical biochemistry·2026
Same journal

Metabolomics approach using UHPLC/QE-MS for the mechanism of He Xue Ming Mu tablets on non-proliferative diabetic retinopathy.

Analytical biochemistry·2026
Same journal

UniRES-GO: Unified residue-level early fusion of sequence and predicted structure for protein function prediction.

Analytical biochemistry·2026
Same journal

IgG detection by enzyme-linked mass spectrometric assay versus color, fluorescent, ECL in buffer and serum.

Analytical biochemistry·2026
See all related articles

Area of Science:

  • Biochemistry
  • Separation Science
  • Materials Science

Background:

  • Chromatography is a critical technique for biomolecule separation.
  • Existing adsorbents like hydroxyapatite have limitations.
  • Development of new, efficient chromatographic media is essential.

Purpose of the Study:

  • To develop a new gel adsorbent for chromatography.
  • To characterize the preparation method and chromatographic properties of calcium tartrate gel.
  • To evaluate the binding capacity of the new adsorbent for biomolecules.

Main Methods:

  • Calcium tartrate crystals were synthesized by varying temperature (10-65°C) and salt concentrations (0.3-3.0 M).
  • Crystals were treated with potassium phosphate (0.075-1.5 M) at 80-100°C and pH 8.5-9.0.

Related Experiment Videos

  • Chromatographic properties, including flow rate and binding capacity for BSA, RNA, and DNA, were assessed.

    • Main Results:

    • Calcium tartrate gel adsorbent particles with sizes ranging from 35-200 microns were prepared.
    • The adsorbent demonstrated flow rates of 25-150 ml/h, dependent on particle size.
    • Binding capacity for BSA, RNA, and DNA was comparable to hydroxyapatite.

    Conclusions:

    • A new chromatographic adsorbent, calcium tartrate gel, has been successfully prepared.
    • The adsorbent exhibits favorable chromatographic properties and binding capacity.
    • The spherical shape of the gel particles allows for efficient column packing.