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Related Experiment Video

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Intermediate filaments enable pathogen docking to trigger type 3 effector translocation.

Brian C Russo1,2, Luisa M Stamm1,2, Matthijs Raaben2

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Nature Microbiology
|August 31, 2016
PubMed
Summary
This summary is machine-generated.

Bacterial type 3 secretion systems (T3SSs) use the intermediate filament vimentin for effector translocation. Vimentin enables stable bacterial docking, a crucial step for effector secretion during infection.

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Area of Science:

  • Microbiology
  • Cell Biology
  • Pathogenesis

Background:

  • Type 3 secretion systems (T3SSs) are critical virulence factors used by bacterial pathogens to inject effector proteins into host cells.
  • Effector translocation requires the formation of a translocon pore across the host cell plasma membrane.

Purpose of the Study:

  • To identify host factors involved in T3SS-mediated effector translocation.
  • To elucidate the role of intermediate filaments in bacterial pathogenesis.

Main Methods:

  • Genome-wide selection screen to identify host factors required for Shigella flexneri infection.
  • Co-immunoprecipitation assays to study protein interactions.
  • Microscopy to assess bacterial docking and effector secretion.

Main Results:

  • The intermediate filament vimentin was identified as essential for Shigella flexneri infection.
  • Vimentin is required for efficient effector translocation by multiple T3SS pathogens, including S. flexneri, Salmonella, and Yersinia.
  • Vimentin interacts with the Shigella translocon protein IpaC and is necessary for stable bacterial docking, which triggers effector secretion.

Conclusions:

  • Stable bacterial docking, mediated by vimentin, is a distinct process from translocon pore formation.
  • Intermediate filaments are essential prerequisites for T3SS-mediated effector secretion and subsequent host cell manipulation.