Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

3.0K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
3.0K
Cryo-electron Microscopy01:28

Cryo-electron Microscopy

4.5K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
4.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sex differences in cardiovascular responses to orthostatic challenge in healthy older persons: A pilot study.

Physiology international·2019
Same author

Color-Doppler sonographic tissue perfusion measurements reveal significantly diminished renal cortical perfusion in kidneys with vesicoureteral reflux.

Indian journal of nephrology·2016
Same author

[Women's work during the Cold War].

Sozialwissenschaftliche Informationen·2012
Same author

Role of variant Creutzfeldt-Jakob disease for safety of treatment with blood components: screening of lymphatic tissue is a potential tool for risk assessment.

European journal of haematology·2003
Same author

CYP2D6 genotyping as an alternative to phenotyping for determination of metabolic status in a clinical trial setting.

AAPS pharmSci·2001
Same author

Clozapine-induced agranulocytosis and hereditary polymorphisms of clozapine metabolizing enzymes: no association with myeloperoxidase and cytochrome P4502D6.

Pharmacopsychiatry·2001

Related Experiment Video

Updated: Mar 15, 2026

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction
12:38

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction

Published on: August 9, 2011

17.9K

Cryo-EM Structure Determination Using Segmented Helical Image Reconstruction.

S A Fromm1, C Sachse1

  • 1EMBL-European Molecular Biology Laboratory, Structural and Computational Biology Unit, Heidelberg, Germany.

Methods in Enzymology
|August 31, 2016
PubMed
Summary
This summary is machine-generated.

Advancements in cryo-electron microscopy (cryo-EM) enable near-atomic resolution of helical assemblies. Optimized image processing and new hardware significantly improve the structure determination of viruses and filaments.

Keywords:
Direct electron detectorsHelical assembliesHelical symmetryNear-atomic resolutionSegmented helical reconstructionSingle-particle helical reconstructionTobacco mosaic virus

More Related Videos

Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins
09:09

Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins

Published on: August 17, 2022

2.9K
Helical Organization of Blood Coagulation Factor VIII on Lipid Nanotubes
12:24

Helical Organization of Blood Coagulation Factor VIII on Lipid Nanotubes

Published on: June 3, 2014

12.8K

Related Experiment Videos

Last Updated: Mar 15, 2026

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction
12:38

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction

Published on: August 9, 2011

17.9K
Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins
09:09

Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins

Published on: August 17, 2022

2.9K
Helical Organization of Blood Coagulation Factor VIII on Lipid Nanotubes
12:24

Helical Organization of Blood Coagulation Factor VIII on Lipid Nanotubes

Published on: June 3, 2014

12.8K

Area of Science:

  • Structural biology
  • Biophysics
  • Microscopy

Background:

  • Helical reconstruction is crucial for determining the structure of viruses and filaments.
  • Classical Fourier-Bessel reconstruction has limitations with flexible or disordered specimens.

Purpose of the Study:

  • To review advancements in cryo-EM techniques for helical assemblies.
  • To highlight the impact of hardware and software improvements on structure determination.

Main Methods:

  • Segmented helical reconstruction treating helices as single particles.
  • Combination of Fourier and real-space image processing.
  • Utilizing direct electron detectors for enhanced image quality.

Main Results:

  • Achieved near-atomic resolution structures of various helical assemblies.
  • Enabled structure determination for specimens previously resistant to classical methods.
  • Segmented helical reconstruction is now a highly productive cryo-EM method.

Conclusions:

  • Latest hardware and optimized image processing have revolutionized helical reconstruction in cryo-EM.
  • The combination of Fourier and real-space methods refines symmetry estimation for high-resolution results.
  • Segmented helical reconstruction is a powerful tool for structural biology.