Related Experiment Video
Updated: Mar 15, 2026
10:36
Rare Event Detection Using Error-corrected DNA and RNA Sequencing
Published on: August 3, 2018
12.7K
DNA Sequencing in Cultural Heritage
Stefania Vai1, Martina Lari2, David Caramelli2
1Department of Biology, University of Florence, Via del Proconsolo 12, 50122, Florence, Italy. stefania.vai@unifi.it.
Topics in Current Chemistry (Cham)
|August 31, 2016
Summary
DNA analysis of degraded samples is crucial for anthropology and evolution. Recent advancements allow ancient genome recovery, enhancing our understanding of the past.
Area of Science:
- Paleogenetics
- Molecular Evolution
- Anthropology
Background:
- DNA analysis of degraded samples has become vital across multiple scientific disciplines.
- Applications include species identification, individual identification, and historical/evolutionary reconstructions.
Observation:
- Significant methodological advancements in recent years have revolutionized paleogenetics.
- These improvements enable the recovery of complete ancient genomes from highly degraded samples.
Findings:
- Ancient DNA (aDNA) analysis can now trace back 400,000 years in temperate regions and 700,000 years in permafrost.
- Even samples subjected to harsh biochemical treatments can be analyzed.
Implications:
- Paleogenetics plays a key role in enhancing cultural heritage through scientific investigation.
- This review synthesizes current methodologies and case studies in degraded DNA analysis.
Related Concept Videos
01:57Sanger Sequencing
777.3K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
777.3K
03:21RNA-seq
12.4K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
12.4K
03:00Next-generation Sequencing
100.6K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
100.6K
01:29Modern Molecular Taxonomy
815
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
815
01:05Maxam-Gilbert Sequencing
13.5K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
Challenges of the Maxam-Gilbert Method
The...
13.5K
02:54Evolutionary Relationships through Genome Comparisons
7.2K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
7.2K