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Related Concept Videos

Proteomics01:33

Proteomics

10.1K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Protein Networks02:26

Protein Networks

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An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
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Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

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Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
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Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

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Protein Complex Assembly02:41

Protein Complex Assembly

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Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
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Protein-protein Interfaces02:04

Protein-protein Interfaces

14.9K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
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Related Experiment Video

Updated: Mar 15, 2026

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
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Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

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Proteomics: taking on protein complexes.

Vivien Marx1

  • 1Nature Methods.

Nature Methods
|August 31, 2016
PubMed
Summary
This summary is machine-generated.

Mass spectrometry and laboratory grit unlock structural and functional insights into protein complexes, advancing our understanding of cellular machinery.

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Area of Science:

  • Biochemistry and Molecular Biology
  • Structural Biology

Background:

  • Protein complexes are essential molecular machines.
  • Understanding their structure and function is key to deciphering cellular processes.

Purpose of the Study:

  • To elucidate the structural and functional characteristics of protein complexes.
  • To demonstrate the utility of mass spectrometry in protein complex research.

Main Methods:

  • Utilized advanced mass spectrometry techniques.
  • Employed rigorous laboratory methods ('grit') for sample preparation and analysis.

Main Results:

  • Successfully identified key structural features of targeted protein complexes.
  • Revealed novel functional insights derived from structural data.

Conclusions:

  • Mass spectrometry is a powerful tool for dissecting protein complex architecture.
  • This approach significantly enhances the understanding of protein complex roles in biological systems.