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Localization and significance of fibronectin in peri-implantation mouse embryos
This study was performed with the aim of clarifying differences in the localization of fibronectin between embryos developed in vivo and in vitro together with the significance of fibronectin in embryos at implantation. Localization of fibronectin in the embryos was first found in the inner cell mass of the early blastocyst in utero (in vivo group). In contrast, when embryos were cultured in vitro from the 2-cell to the blastocyst stage (in vitro group), fibronectin could not be observed in the late blastocyst. When the blastocysts developed either in vivo or in vitro were further culture for 3 days in Dulbecco's Modified Eagle Medium (D'MEM) containing 10% newborn calf serum, the in vitro implantation (trophoblastic outgrowth in vitro) rates were 90% and 37%, respectively. If the blastocysts developed in vivo were cultured in D'MEM alone, the in vitro implantation rate was as low 29%. However, if fibronectin was added to the D'MEM, instead of newborn calf serum, the in vitro implantation rates were improved. These results led to the conclusion that fibronectin is necessary for the implantation of blastocysts.
This study was performed with the aim of clarifying differences in the localization of fibronectin between embryos developed in vivo and in vitro together with the significance of fibronectin in embryos at implantation. Localization of fibronectin in the embryos was first found in the inner cell mass of the early blastocyst in utero (in vivo group). In contrast, when embryos were cultured in vitro from the 2-cell to the blastocyst stage (in vitro group), fibronectin could not be observed in the late blastocyst. When the blastocysts developed either in vivo or in vitro were further culture for 3 days in Dulbecco's Modified Eagle Medium (D'MEM) containing 10% newborn calf serum, the in vitro implantation (trophoblastic outgrowth in vitro) rates were 90% and 37%, respectively. If the blastocysts developed in vivo were cultured in D'MEM alone, the in vitro implantation rate was as low 29%. However, if fibronectin was added to the D'MEM, instead of newborn calf serum, the in vitro implantation rates were improved. These results led to the conclusion that fibronectin is necessary for the implantation of blastocysts.