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Preparation of drug-in-cyclodextrin-in-liposomes at a large scale using a membrane contactor: Application to
Riham Gharib1, Hélène Greige-Gerges2, Alia Jraij2
1Bioactive Molecules Research Group, Faculty of Sciences, Doctoral School of Sciences and Technologies, Lebanese University, Lebanon; Laboratoire d'Automatique et de Génie des Procédés, Université Claude Bernard Lyon 1, France.
Abstract:
The present study aimed to prepare liposomes loaded with cyclodextrin/drug inclusion complexes at a pilot scale based on the ethanol injection technique. Anethole (ANE), a major component of anise and fennel essential oils, was used as a model of a volatile and highly hydrophobic drug. Membrane contactor (600mL) and a pilot plant (3L) were used for liposome production. The liposome preparations obtained were characterized for size, polydispersity index, zeta potential, morphology, stability and ANE release rate. All experimental set-ups were shown to be appropriate for the preparation of small, multilamellar vesicles with narrow size distribution and good stability at 4°C. The drug release study showed that only a small amount of ANE was released from liposome formulations after 21days of storage at 4°C. The loading rate of ANE was higher when ethanol was evaporated directly on the pilot plant compared to a rotary evaporation.