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Updated: Mar 15, 2026
High Fat Diet Feeding and High Throughput Triacylglyceride Assay in Drosophila Melanogaster
Published on: September 13, 2017
High-Fat Diet Induces Oxidative Stress and MPK2 and HSP83 Gene Expression in Drosophila melanogaster
Mariane Trindade de Paula1, Márcia Rósula Poetini Silva1, Stífani Machado Araujo1
1Laboratório de Avaliações Farmacológicas e Toxicológicas Aplicadas às Moléculas Bioativas, Universidade Federal do Pampa (Unipampa), Campus Itaqui, 97650-000 Itaqui, RS, Brazil.
Abstract:
The consumption of a high-fat diet (HFD) causes alteration in normal metabolism affecting lifespan of flies; however molecular mechanism associated with this damage in flies is not well known. This study evaluates the effects of ingestion of a diet supplemented with 10% and 20% of coconut oil, which is rich in saturated fatty acids, on oxidative stress and cells stress signaling pathways. After exposure to the diet for seven days, cellular and mitochondrial viability, lipid peroxidation and antioxidant enzymes SOD and CAT activity, and mRNA expression of antioxidant enzymes HSP83 and MPK2 were analyzed. To confirm the damage effect of diet on flies, survival and lifespan were investigated. The results revealed that the HFD augmented the rate of lipid peroxidation and SOD and CAT activity and induced a higher expression of HSP83 and MPK2 mRNA. In parallel, levels of enzymes involved in lipid metabolism (ACSL1 and ACeCS1) were increased. Our data demonstrate that association among metabolic changes, oxidative stress, and protein signalization might be involved in shortening the lifespan of flies fed with a HFD.
