Related Experiment Video
Updated: Mar 15, 2026

Chessboard-like Burn Wound Healing Model of Mice Based on Digital Heating Device
Published on: December 27, 2024
Keratinocyte growth factor and the expression of wound-healing-related genes in primary human keratinocytes from burn
Verônica Chomiski1, Alfredo Gragnani2, Jéssica Bonucci3
1Fellow MSc degree, Division of Plastic Surgery, Department of Surgery, Universidade Federal de São Paulo (UNIFESP0, Sao Paulo-SP, Brazil. Acquisition and interpretation of data, technical procedures, manuscript writing.
Purpose:
To evaluate the effect of keratinocyte growth factor (KGF) treatment on the expression of wound-healing-related genes in cultured keratinocytes from burn patients.
Methods:
Keratinocytes were cultured and divided into 4 groups (n=4 in each group): TKB (KGF-treated keratinocytes from burn patients), UKB (untreated keratinocytes from burn patients), TKC (KGF-treated keratinocytes from controls), and UKC (untreated keratinocytes from controls). Gene expression analysis using quantitative polymerase chain reaction (qPCR) array was performed to compare (1) TKC versus UKC, (2) UKB versus UKC, (3) TKB versus UKC, (4) TKB versus UKB, (5) TKB versus TKC, and (6) UKB versus TKC.
Results:
Comparison 1 showed one down-regulated and one up-regulated gene; comparisons 2 and 3 resulted in the same five down-regulated genes; comparison 4 had no significant difference in relative gene expression; comparison 5 showed 26 down-regulated and 7 up-regulated genes; and comparison 6 showed 25 down-regulated and 11 up-regulated genes.
Conclusion:
There was no differential expression of wound-healing-related genes in cultured primary keratinocytes from burn patients treated with keratinocyte growth factor.
Related Concept Videos
Clinical Applications of Epidermal Stem Cells
Renewal of Skin Epidermal Stem Cells
Phases of Wound Repair
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
Overview of Regeneration and Repair
Regeneration
All animals have varying degrees of...
Burn Injuries
The damage results in the death of skin cells, which can lead to a massive loss of fluid. Dehydration, electrolyte imbalance, and renal and circulatory failure follow, which can be fatal. Burn patients are treated with intravenous fluids to offset...

