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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Mar 15, 2026

Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors

Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors

Published on: June 7, 2018

Analyzing the Tumor Microenvironment by Flow Cytometry.

Yoon Kow Young¹, Alicia M Bolt¹, Ryuhjin Ahn¹

¹Lady Davis Institute for Medical Research, McGill University, Montreal, QC, Canada.

Methods in Molecular Biology (Clifton, N.J.)

|September 2, 2016

Summary

Flow cytometry is vital for tumor microenvironment research, enabling rapid cell identification. This guide covers instrumentation, panel design, protocols for lymphoid tissue and solid tumors, and data analysis.

Keywords:

Compensation Flow cytometry (FCM)Fluorescence minus one (FMO)Fluorescence-activated cell sorting (FACS)Immune cell infiltrate Intracellular staining Isotype control Multi-parameter Solid tumor Spillover Staining panel Tumor microenvironment

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Last Updated: Mar 15, 2026

Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors

Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors

Published on: June 7, 2018

Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model

Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model

Published on: April 13, 2015

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells

Published on: November 28, 2015

Area of Science:

Immunology
Cell Biology
Biotechnology

Background:

The tumor microenvironment (TME) is complex, requiring advanced techniques for cellular analysis.
Flow cytometry offers high-throughput quantification and identification of diverse cell populations within heterogeneous samples.

Purpose of the Study:

To provide an overview of flow cytometry instrumentation.
To detail considerations and steps for designing effective flow cytometry staining panels.
To present protocols for analyzing lymphoid tissues and solid tumor leukocyte infiltrates.

Main Methods:

Overview of flow cytometry principles and instrumentation.
Guidance on multicolor panel design for TME analysis.
Detailed staining protocols for specific sample types (lymphoid tissue, solid tumors).
Exemplification of flow cytometry data analysis.

Main Results:

Established protocols facilitate robust characterization of immune cells within the TME.
Effective panel design is crucial for accurate cell identification and quantification.
Data analysis strategies enable comprehensive understanding of cellular composition.

Conclusions:

Flow cytometry is an indispensable tool for TME research.
Standardized protocols and thoughtful panel design enhance the reliability of flow cytometry studies.
This work provides a practical framework for TME cellular analysis using flow cytometry.