Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A multi-center study on the consistency of drug sensitivity testing in patients with acute myeloid leukemia.

NPJ precision oncology·2026
Same author

Spatial artifact detection improves the reproducibility of drug screening experiments.

iScience·2025
Same author

High-content Image-based Drug Testing of Patients' Primary Fibroblasts Reveals Potential New Treatment Options for Localized Scleroderma.

Acta dermato-venereologica·2025
Same author

Functional screening identifies kinesin spindle protein inhibitor filanesib as a potential treatment option for hepatoblastoma.

NPJ precision oncology·2025
Same author

Computational drug repurposing: approaches, evaluation of in silico resources and case studies.

Nature reviews. Drug discovery·2025
Same author

Characterisation of new in vitro models and identification of potentially active drugs in angiosarcoma.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2024

Related Experiment Video

Updated: Mar 15, 2026

Genome-wide RNAi Screening to Identify Host Factors That Modulate Oncolytic Virus Therapy
08:51

Genome-wide RNAi Screening to Identify Host Factors That Modulate Oncolytic Virus Therapy

Published on: April 3, 2018

9.6K

High Throughput siRNA Screening Using Reverse Transfection.

Carina von Schantz1, Jani Saarela2

  • 1Institute for Molecular Medicine Finland (FIMM), University of Helsinki, Tukholmankatu 8, Helsinki, FI-00290, Finland. carina.vonschantz-fant@helsinki.fi.

Methods in Molecular Biology (Clifton, N.J.)
|September 2, 2016
PubMed
Summary
This summary is machine-generated.

This study presents a high-throughput screening method for gene silencing in breast cancer cells using small interfering RNA (siRNA). The research details techniques for assessing cell viability and death in MDA-MB-231 cells.

Keywords:
Acoustic droplet ejectionGene silencingHigh throughput screeningRNAiReverse transfectionsiRNA screening

More Related Videos

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
07:52

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

Published on: November 2, 2020

7.2K
Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
11:32

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

7.5K

Related Experiment Videos

Last Updated: Mar 15, 2026

Genome-wide RNAi Screening to Identify Host Factors That Modulate Oncolytic Virus Therapy
08:51

Genome-wide RNAi Screening to Identify Host Factors That Modulate Oncolytic Virus Therapy

Published on: April 3, 2018

9.6K
In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
07:52

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

Published on: November 2, 2020

7.2K
Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
11:32

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

7.5K

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Genetics

Background:

  • RNA interference (RNAi) is a vital tool for reducing gene expression.
  • Gene silencing is crucial for understanding gene function in diseases like breast cancer.
  • The MDA-MB-231 cell line is a widely used model for triple-negative breast cancer research.

Purpose of the Study:

  • To develop and describe a high-throughput screening method for siRNA-mediated gene silencing.
  • To optimize gene knockdown in the MDA-MB-231 breast cancer cell line.
  • To establish robust methods for assessing cell viability and death following gene silencing.

Main Methods:

  • High-throughput screening utilizing reverse transfection for siRNA delivery.
  • Application of small interfering RNA (siRNA) for targeted gene knockdown.
  • Implementation of homogenous assays and image-based analysis for cell viability and death detection.

Main Results:

  • A scalable method for efficient gene silencing in MDA-MB-231 cells was established.
  • Two distinct, validated assays for quantifying cell viability and death were successfully implemented.
  • The screening approach facilitates rapid assessment of gene function in breast cancer models.

Conclusions:

  • The described high-throughput RNA interference method enables efficient gene silencing in breast cancer cells.
  • The developed cell viability and death assays are suitable for screening applications.
  • This approach provides a powerful tool for functional genomics in cancer research.