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Related Concept Videos

RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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Performing Custom MicroRNA Microarray Experiments
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Self-Assembled Cell Microarray (SAMcell) for High-Throughput RNAi Screening.

Hanshuo Zhang1, Juan Li2

  • 1Biomedical Engineering Department, College of Engineering, Peking University, Beijing, 100871, China. shzzhshuo@sina.com.

Methods in Molecular Biology (Clifton, N.J.)
|September 2, 2016
PubMed
Summary

Self-assembled cell microarray (SAMcell) offers a streamlined approach to RNA interference (RNAi) screening. This novel method enables high-throughput investigation of gene functions in cellular processes like migration and proliferation.

Keywords:
Cell behaviorChipHigh-throughput screeningRNAiSAMcell

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Cell Biology

Background:

  • RNA interference (RNAi) is a key research tool for high-throughput screening.
  • Traditional RNAi methods utilize multi-well plates, requiring expensive equipment and complex procedures.

Purpose of the Study:

  • To introduce a novel method, self-assembled cell microarray (SAMcell), for cell-based high-throughput screening.
  • To overcome the limitations of traditional RNAi screening methods.

Main Methods:

  • Integration of micro-fabrication, reverse transfection, and RNAi technologies.
  • Development of a cell chip for direct cell behavior investigations.
  • Application of SAMcell for large-scale functional screening assays.

Main Results:

  • Successful implementation of SAMcell for investigating gene modulators.
  • Identification of genes influencing cell migration.
  • Identification of genes influencing cell proliferation and apoptosis.

Conclusions:

  • SAMcell provides an efficient and cost-effective platform for RNAi-based functional screening.
  • This technology facilitates the study of gene function in various cellular processes.
  • SAMcell advances the field of high-throughput cell-based assays.