The effect of chronic alcohol consumption on mitochondrial calcium handling in hepatocytes

The damage to liver mitochondria is universally observed in both humans and animal models after excessive alcohol consumption. Acute alcohol treatment has been shown to stimulate calcium (Ca²⁺) release from internal stores in hepatocytes. The resultant increase in cytosolic Ca²⁺ is expected to be accumulated by neighboring mitochondria, which could potentially lead to mitochondrial Ca²⁺ overload and injury. Our data indicate that total and free mitochondrial matrix Ca²⁺ levels are, indeed, elevated in hepatocytes isolated from alcohol-fed rats compared with their pair-fed control littermates. In permeabilized hepatocytes, the rates of mitochondrial Ca²⁺ uptake were substantially increased after chronic alcohol feeding, whereas those of mitochondrial Ca²⁺ efflux were decreased. The changes in mitochondrial Ca²⁺ handling could be explained by an up-regulation of the mitochondrial Ca²⁺ uniporter and loss of a cyclosporin A-sensitive Ca²⁺ transport pathway. In intact cells, hormone-induced increases in mitochondrial Ca²⁺ declined at slower rates leading to more prolonged elevations of matrix Ca²⁺ in the alcohol-fed group compared with controls. Moreover, treatment with submaximal concentrations of Ca²⁺-mobilizing hormones markedly increased the levels of mitochondrial reactive oxygen species (ROS) in hepatocytes from alcohol-fed rats, but did not affect ROS levels in controls. The changes in mitochondrial Ca²⁺ handling are expected to buffer and attenuate cytosolic Ca²⁺ increases induced by acute alcohol exposure or hormone stimulation. However, these alterations in mitochondrial Ca²⁺ handling may also lead to Ca²⁺ overload during cytosolic Ca²⁺ increases, which may stimulate the production of mitochondrial ROS, and thus contribute to alcohol-induced liver injury.