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A new micro swim-up procedure for sperm preparation in ICSI treatments: preliminary microbiological testing
Simone Palini1, Mariangela Primiterra1, Silvia De Stefani1
1Cervesi Hospital, IVF Unit, AUSL Romagna, Italy.
Objective:
This study aimed to assess the levels of microbial contamination in semen samples before and after the micro swim-up (MSU) procedure in intra-cytoplasmic sperm injection (ICSI). The new method is an upgrade to the classic wash swim-up procedure.
Methods:
Semen analysis and microbiological tests were carried out before and after the MSU procedure. A total of twenty semen samples were analyzed.
Results:
Pathogens were observed in semen samples only before MSU and never after ICSI. Microbiological tests revealed a large prevalence of gram-positive cocci [Staphylococcus spp. (n=16, 80%) and viridans streptococci (n=10, 50%)]. The results of this study indicate that direct MSU in ICSI improved the ICSI workflow.
Conclusion:
The new workflow is faster and more affordable, and is likely to prevent infection problems that could arise from the normal microbial flora of the semen.
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