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Related Experiment Videos

Effect of counting errors on immunoassay precision.

G G Klee1, G Post

  • 1Department of Laboratory Medicine and Pathology, Mayo Foundation, Rochester 55905.

Clinical Chemistry
|July 1, 1989
PubMed
Summary
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Gamma scintillation counting error impacts radioimmunoassays (RIA) and immunoradiometric assays (IRMA). Increasing maximum counts bound to 10,000 improves assay precision, especially at low analyte concentrations.

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Medical Diagnostics

Background:

  • Gamma scintillation counting is a critical component in radioimmunoassays (RIA) and immunoradiometric assays (IRMA).
  • Understanding the impact of counting error is essential for accurate analyte concentration determination.

Purpose of the Study:

  • To analyze how gamma scintillation counting error propagates into analyte concentration estimates.
  • To evaluate the effect of maximum counts bound on assay precision in RIA and IRMA.

Main Methods:

  • Mathematical analysis and computer simulation were employed.
  • Empirical derivation of parameters for logit-log data-reduction models for digoxin, triiodothyronine (RIA), and ferritin (IRMA).

Main Results:

Related Experiment Videos

  • Counting error, expressed as a coefficient of variation (CV) of analyte concentration, decreased with the inverse of the square root of maximum counts bound.
  • Higher counting-error CVs were observed at lower analyte concentrations for both RIA and IRMA.
  • Assay CVs significantly decreased as maximum counts bound increased from 500 to 10,000, with diminishing returns beyond that.

Conclusions:

  • RIA and IRMA systems utilizing duplicate determinations with at least 10,000 maximum counts bound offer adequate precision.
  • Potential precision limitations may still exist at very low analyte concentrations even with high counts bound.