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Related Experiment Video

Updated: Mar 15, 2026

The Synthesis, Characterization and Reactivity of a Series of Ruthenium N-triphosPh Complexes

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Chemical Posttranslational Modification with Designed Rhodium(II) Catalysts.

S C Martin¹, M B Minus¹, Z T Ball¹

¹Rice University, Houston, TX, United States.

Methods in Enzymology

|September 3, 2016

Summary

Rhodium(II) catalysts mimic natural enzymes, enabling selective protein modification using molecular recognition. This breakthrough offers new tools for studying protein interactions and creating novel protein conjugates.

Keywords:

Bioconjugation Diazo Enzyme design Protein modification Rhodium SH3 domain

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Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry

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Published on: August 19, 2012

Area of Science:

Biochemistry
Organic Chemistry
Chemical Biology

Background:

Natural enzymes exhibit high selectivity in modifying biomolecules.
Molecular recognition is key to enzyme specificity.
Developing synthetic catalysts with similar selectivity is a significant challenge.

Purpose of the Study:

To develop synthetic catalysts that mimic enzymatic selectivity for protein modification.
To explore the use of rhodium(II) catalysts with diazo reagents for protein functionalization.
To assess the applicability of this method in complex biological environments.

Main Methods:

Utilized rhodium(II) catalysts for selective protein modification.
Employed functionalized diazo reagents for targeted chemical transformations.
Tested the catalytic system across diverse protein folds and in cell lysate.
Assessed modification efficiency using a protein blot method.

Main Results:

Rhodium(II) catalysts demonstrated molecular recognition for selective protein modification.
The catalytic system proved effective on various protein structures.
Successful application was achieved in complex environments like cell lysate.
A simple protein blot method accurately quantified modification.

Conclusions:

Rhodium(II) catalysts offer a novel tool for probing protein-binding events.
This methodology provides a new synthetic route to protein conjugates.
Potential applications include medical, biochemical, and materials science.