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Updated: Mar 15, 2026

Single-Particle Cryo-EM Data Collection with Stage Tilt using Leginon
Published on: July 1, 2022
Tilting and moving-object lens for a 3D electron microscope
1Honorary Member, The Japanese Society of Microscopy, 1-14-9 Takakurachou, Miyakojima-ku, Osaka 534-0011, Japan ura_katsumi@mvb.biglobe.ne.jp.
Abstract:
I investigated the tilting and movement of the objective lens of a 3D electron microscope electrically as an extension of the moving-objective lens concept. The electric or magnetic potential along the tilted optical axis is analytically expressed by a multipole potential expansion about the fixed central axis. The field distributions for axially symmetric dipole and quadrupole components are numerically shown, where the optical axis of a bell-shaped magnetic lens is tilted around the lens center by up to 60°. The hexapole and octapole components are also shown at a tilt angle of 45°.
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