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Functional Profiling Using the Saccharomyces Genome Deletion Project Collections.

Corey Nislow1, Lai Hong Wong1, Amy Huei-Yi Lee1

  • 1Department of Pharmaceutical Sciences, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada.

Cold Spring Harbor Protocols
|September 3, 2016
PubMed
Summary
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The yeast deletion collections enable comprehensive fitness measurements for all genes. These functional genomic protocols detail how to use these collections for high-throughput analysis of gene function and biological insights.

Area of Science:

  • Genomics
  • Molecular Biology
  • Yeast Genetics

Background:

  • Traditional "omic" methods offer limited insight into whole-organism fitness.
  • The Saccharomyces Genome Deletion Project created comprehensive yeast deletion collections.
  • These collections are invaluable for systematic gene function analysis.

Purpose of the Study:

  • To present functional genomic protocols for utilizing yeast deletion collections.
  • To detail methods for maintaining, propagating, and storing these collections.
  • To describe growth fitness assays for single and parallel screening platforms.

Main Methods:

  • Utilizing yeast deletion collections with unique 20-bp molecular barcodes.
  • Implementing protocols for strain maintenance and propagation.

Related Experiment Videos

  • Performing high-throughput growth fitness assays for phenotypic analysis.

    • Main Results:

    • Established protocols for the systematic and parallel analysis of gene functions.
    • Demonstrated the utility of molecular barcodes for massively parallel fitness analysis.
    • Provided insights into biological functions, drug mechanisms, and stress responses.

    Conclusions:

    • Yeast deletion collections are a powerful tool for functional genomics.
    • The presented protocols facilitate high-throughput fitness assays.
    • These methods offer broad applications in understanding gene function and biological processes.