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The involvement of proline-rich protein Mus musculus predicted gene 4736 in ocular surface functions

Xia Qi1, Sheng-Wei Ren2, Feng Zhang3

  • 1State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong Academy of Medical Sciences, Qingdao 266071, Shandong Province, China.

International Journal of Ophthalmology
|September 3, 2016
PubMed
Abstract

Insights

This study investigated Mus musculus predicted gene 4736 (MP4) and proline-rich protein BstNI subfamily 1 (Prb1) in ocular surface responses to infection. MP4 expression increased in mouse corneas during Pseudomonas aeruginosa keratitis, suggesting a role in ocular surface homeostasis.

Area of Science:

  • Ocular Surface Biology
  • Immunology
  • Molecular Biology

Background:

  • Proline-rich proteins are crucial for tissue structure and defense.
  • Mus musculus predicted gene 4736 (MP4) and proline-rich protein BstNI subfamily 1 (Prb1) were upregulated in corneal organ cultures during fungal exposure.
  • Their role in the ocular surface, particularly during infection, requires elucidation.

Purpose of the Study:

  • To confirm the expression of MP4 and Prb1 in the ocular surface.
  • To investigate the potential functions of MP4 and Prb1 in ocular surface physiology and pathology.
  • To assess MP4 expression during bacterial keratitis.

Main Methods:

  • Established a Pseudomonas aeruginosa keratitis model in Balb/c mice.
  • Quantified MP4 mRNA levels using real-time PCR.
  • Detected MP4 protein using immunohistochemistry (IHC) and Western blot.
  • Analyzed MP4 expression in lacrimal glands and screened public data for other expression sites using BioGPS.

Main Results:

  • MP4 mRNA and protein levels were significantly upregulated in mouse corneas one day post-keratitis induction.
  • Immunohistochemistry confirmed MP4 presence in normal murine lacrimal glands.
  • Bioinformatics analysis indicated abundant MP4 expression in lacrimal glands.

Conclusions:

  • MP4 and Prb1 are implicated in the physiological and pathological processes of the ocular surface.
  • These genes may contribute to ocular surface homeostasis, especially in conditions like dry eye.
  • Further research is warranted to explore their functional roles and disease correlations.
Keywords:
Mus musculus predicted gene 4736Pseudomonas aeruginosa keratitisocular surfaceproline-rich protein

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